Human Hemopexin ELISA Kit

Catalog number: KE00101

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$399.00

Freight/Packing £00.00

Size: 09µ/gram

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Overview


Product name:
Human Hemopexin ELISA Kit

Tests:
1 X 96 well plate

Sample type:
Plasma, Serum ,Cell culture supernatant

Assay type:
Sandwich

Sensitivity:
12.5 ng/mL

Range:
62.5 - 4000 ng/mL

Reacted Species:
Human

Tested applications:
Sandwich ELISA

Recovery:
Sample TypeAverageRange
Plasma 99% 78%-121%
Cell culture supernatant 93% 77%-121%

Product overview:
KE00101 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Hemopexin ELISA kit is to be used to detect and quantify protein levels of endogenous Hemopexin. The assay recognizes human Hemopexin. A polyclonal antibody specific for Hemopexin has been pre-coated onto the microwells. The Hemopexin protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for Hemopexin is added to detect the captured Hemopexin protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

Properties


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

Synonyms:
Beta 1B glycoprotein, hemopexin, HPX
Background

Hemopexin (HPX) is the plasma protein responsible for scavenging heme, thus preventing heme-mediated oxidative stress and heme-bound iron loss. In addition, hemopexin blocks heme activation of immune receptors and vascular inflammatory processes. It is mainly expressed in liver, the synthesis of which is induced after inflammation. Alterations of plasma hemopexin level have been linked to disorders like atherosclerosis and inflammatory diseases.


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