Human IL-11 ELISA Kit
Cat no : KE00246
Synonyms
IL11, Adipogenesis inhibitory factor, AGIF, IL 11, interleukin 11
Validation Data Gallery
Product Information
KE00246 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Human IL-11 ELISA kit is to be used to detect and quantify protein levels of endogenous human IL-11. The assay recognizes human IL-11. An antibody specific for human IL-11 has been pre-coated onto the microwells. The human IL-11 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human IL-11 is added to detect the captured human IL-11 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human IL-11 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell cuture supernatants |
| Assay type | Sandwich |
| Sensitivity | 22.95 pg/mL |
| Range | 62.5-4000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3589 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell cuture supernatants | 104% | 95%-111% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 1873.6 | 84.9 | 4.5 |
| 2 | 20 | 442.7 | 16.8 | 3.8 |
| 3 | 20 | 108.2 | 8.2 | 7.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 1842.0 | 102.5 | 5.6 |
| 2 | 24 | 463.7 | 21.9 | 4.7 |
| 3 | 24 | 112.8 | 10.5 | 9.3 |
Background Information
IL-11 is a multifunctional cytokine produced by a variety of cells, which regulates hematopoiesis, bone function and cytoprotecting in the gut. As a member of the glycoprotein (GP) 130 cytokine family, IL-11 can activate the JAK-STAT3 pathway. Its pleiotropic effect is based on both anti-inflammatory and pro-inflammatory effects. The anti-inflammatory effect is downregulating the macrophage function by inhibiting the IL-1β, IL-12 and NO production. The pro-inflammatory effect is inducing the initial CD4+ T cells to differentiate into Th17 cells, and causing the inflammatory response in the multiple sclerosis. IL-11 is related to inflammation and cancer since its transmembrane co-receptor IL-11Rα has been shown to overexpress in lung cancer, colorectal cancer, gastric cancer, breast cancer, prostate cancer and osteosarcoma.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | IL11, Adipogenesis inhibitory factor, AGIF, IL 11, interleukin 11 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.