Human IL-1RL1/ST2 ELISA Kit
Cat no : KE00293
Synonyms
DER4, FIT 1, IL1RL1, interleukin 1 receptor like 1, Protein ST2, ST2, ST2L, ST2V, T1
Validation Data Gallery
Product Information
KE00293 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL-1RL1/ST2 ELISA kit is to be used to detect and quantify protein levels of endogenous IL-1RL1/ST2. The assay recognizes human IL-1RL1/ST2. An antibody specific for IL-1RL1/ST2 has been pre-coated onto the microwells. The IL-1RL1/ST2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for IL-1RL1/ST2 is added to detect the captured IL-1RL1/ST2 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human IL-1RL1/ST2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 5.6 pg/mL |
| Range | 31.3-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 9173 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 106% | 88%-131% |
| Cell culture supernatant | 94% | 84%-103% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 954.4 | 41.1 | 4.3 |
| 2 | 20 | 234.8 | 9.1 | 3.9 |
| 3 | 20 | 52.3 | 2.3 | 4.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 1,041.2 | 43.2 | 4.1 |
| 2 | 24 | 246.7 | 8.6 | 3.5 |
| 3 | 24 | 56.0 | 3.2 | 5.8 |
Background Information
Protein ST2, also known as IL-1RL1, is a member of the interleukin 1 receptor family. The gene of ST2 encodes three splice variants: a soluble secreted form (sST2), a transmembrane receptor form (ST2L), and a variant form of ST2 (ST2V). Interleukin-33 (IL-33) has been identified as a functional ligand of ST2L. IL-33 exerts its cellular functions by binding a receptor complex composed of ST2L and IL-1R accessory protein (IL-1RAcP). The interaction of IL-33 and ST2L activates mitogen-activated protein kinases and several biochemical pathways. The end of these reactions is the activation of the inhibitor of nuclear factor-kB (NF-kB) kinase complex, triggering NF-kB activity. sST2 seems to act as a decoy-receptor for IL-33: it binds IL-33 thus subtracting such a molecule from the interaction with ST2L. sST2 is formed by many cells and sST2 level is increased as response to myocardial stress, as well as in inflammatory conditions, including allergic asthma.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | DER4, FIT 1, IL1RL1, interleukin 1 receptor like 1, Protein ST2, ST2, ST2L, ST2V, T1 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.