Speedy™ Human/Cynomolgus Monkey IL-6 One-Step ELISA Kit
Cat no : SE50001
Synonyms
IL6, B-cell stimulatory factor 2, IFN-beta-2, IL 6, Interferon beta-2
Validation Data Gallery
Product Information
| Product name | Speedy™ Human/Cynomolgus Monkey IL-6 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Human serum, Human plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 1.2 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Human/Cynomolgus Monkey |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3569 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 91% | 83%-100% |
| Human cell culture supernatant | 96% | 89%-109% |
| Cynomolgus monkey cell culture supernatant | 105% | 102%-109% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 246.5 | 3.7 | 1.5 |
| 2 | 8 | 64.0 | 1.7 | 2.6 |
| 3 | 8 | 31.8 | 0.9 | 2.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 246.5 | 4.1 | 1.7 |
| 2 | 16 | 64.3 | 1.3 | 2.0 |
| 3 | 16 | 31.9 | 0.9 | 2.9 |
Background Information
Interleukin-6 (IL6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL6 Plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | IL6, B-cell stimulatory factor 2, IFN-beta-2, IL 6, Interferon beta-2 |
FAQ
1、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data?
Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.