Human IL-23 ELISA Kit
Cat no : KE00088
Synonyms
IL 23, IL 23 A, IL 23 subunit alpha, IL 23A, IL 23p19, IL-23, IL23A, IL-23A, IL23P19, Interleukin 23 subunit alpha, Interleukin 23 subunit p19, P19, SGRF
Validation Data Gallery
Product Information
KE00088 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL23 ELISA kit is to be used to detect and quantify protein levels of endogenous IL23. The assay recognizes human IL23. An antibody specific for IL23 has been pre-coated onto the microwells. The IL23 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for IL23 is added to detect the captured IL23 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human IL-23 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 3.3 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 51561 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 95% | 82%-117% |
| Cell culture supernatants | 96% | 82%-120% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 775.2 | 58.7 | 7.6 |
| 2 | 20 | 180.3 | 15.8 | 8.8 |
| 3 | 20 | 20.0 | 1.2 | 5.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 770.1 | 61.5 | 8.0 |
| 2 | 24 | 166.4 | 15.3 | 9.2 |
| 3 | 24 | 13.7 | 1.2 | 9.0 |
Background Information
Interleukin 23 (IL23) is a member of the IL12 cytokine family and composed of two subunits, IL12p40 and IL23p19. It is produced by antigen presenting cells and has been shown to promote the production and survival of a distinct lineage of T-cells called TH17 cells. A functional receptor for IL23 (the IL23 receptor) has been identified and is composed of IL12Rβ1 and IL23R. IL23 is expressed chiefly by the macrophages and DCs. The IL-23R is found on memory T cells, NKT cells, macrophages, DCs, and naive T cells upon activation by TGF-βand IL6. The main biological effects of IL23 identified initially consist of stimulation of antigen presentation by DCs, T cell differentiation to Th17 cells, and production of interferon-γ (IFN-γ). IL23 acts also as an end-stage effector cytokine through direct action on macrophages.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | IL 23, IL 23 A, IL 23 subunit alpha, IL 23A, IL 23p19, IL-23, IL23A, IL-23A, IL23P19, Interleukin 23 subunit alpha, Interleukin 23 subunit p19, P19, SGRF |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Rheumatology (Oxford) Utility of nailfold capillary assessment for predicting psoriatic arthritis based on a prospective observational cohort study |