Human INS ELISA Kit
Cat no : KE00045
Synonyms
ILPR, INS, IRDN
Validation Data Gallery
Product Information
KE00045 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The INS ELISA kit is to be used to detect and quantify protein levels of endogenous INS. The assay recognizes human INS. An antibody specific for INS has been pre-coated onto the microwells. The INS protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for INS is added to detect the captured INS protein. For signal development, Sterptravidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human INS ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 1.3 pmol/L |
| Range | 7.8 - 500 pmol/L |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3630 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 100% | 82%-123% |
IntraAssay
| Sample | n | mean ( pmol/L) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 295.7 | 27.7 | 9.4 |
| 2 | 20 | 126.0 | 12.0 | 9.5 |
| 3 | 20 | 64.6 | 6.2 | 9.6 |
InterAssay
| Sample | n | mean ( pmol/L) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 314.2 | 24.5 | 7.8 |
| 2 | 24 | 149.2 | 13.2 | 8.8 |
| 3 | 24 | 76.0 | 7.3 | 9.6 |
Background Information
Insulin is a peptide hormone, produced by beta cells of the pancreas, and is central to regulating carbohydrate and fat metabolism in the body. It participates in glucose utilization, protein synthesis and in the formation and storage of neutral lipids. Insulin is synthesized as a precursor molecule, proinsulin, which is processed prior to secretion. A- and B-peptides are joined together by a disulfide bond to form insulin, while the central portion of the precursor molecule is cleaved and released as the C-peptide. Defects in insulin results in type 1 diabetes mellitus.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | ILPR, INS, IRDN |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Front Cardiovasc Med ANGPTL3 Is Involved in the Post-prandial Response in Triglyceride-Rich Lipoproteins and HDL Components in Patients With Coronary Artery Disease. | ||
Exp Clin Endocrinol Diabetes FGF19 Promotes the Proliferation and Insulin Secretion from Human Pancreatic β Cells Via the IRS1/GLUT4 Pathway | ||
Front Pharmacol Lactobacillus reuteri strain 8008 attenuated the aggravation of depressive-like behavior induced by CUMS in high-fat diet-fed mice through regulating the gut microbiota |