Anti-Human IgG1 Rabbit Recombinant Antibody, PBS Only

• 

  IHC staining of human tonsillitis using 98804-3-RR (same clone as 98804-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 98721-9-RR (Human IgG1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 98804-3-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 98804-3-RR (same clone as 98804-3-PBS)

  1x10^6 human PBMCs were stained with 0.25 ug Anti-Human Human IgG1 Rabbit RecAb (98804-3-RR, Clone: 251296E10) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD19 (4G7) Mouse IgG2a Recombinant Antibody (CL647-65562, Clone: 4G7). The cells originate from CD11b-negative cells. Cells were incubated with FC Receptor Block prior to staining. Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019). This data was developed using the same antibody clone with 98804-3-PBS in a different storage buffer formulation.

IgG1 Uni-rAb^® Recombinant Antibody for IHC, FC

Cat No. 98804-3-PBS

Clone No.251296E10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, FC

Synonyms

Human IgG1, IGHG1, Immunoglobulin heavy constant gamma 1

---

Product Information

98804-3-PBS targets IgG1 in IHC, FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg6672

  Product name: recombinant human IgG1-IgK protein

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 10009498 of

  Sequence: /

• Full Name: immunoglobulin heavy constant gamma 1 (G1m marker)
• Gene Symbol: IGHG1
• Gene ID (NCBI): 3500
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Human Immunoglobulin G subclass 1 (IgG1) is the most abundant antibody subclass in human serum, constituting approximately 60-70% of total IgG. It serves as a critical mediator of the adaptive immune system's defense against pathogens. Human IgG1 represents a perfect balance of specificity, stability, and powerful immune-activating capacity. Its dominance in the biotherapeutic landscape is a testament to its versatility and effectiveness. This antibody does not recognize IgA2.