Human LGALS9/Galectin-9 ELISA Kit
Sensitivity
3.9 pg/mL
Range
31.25-2000 pg/mL, 62.5-4000 pg/mL
Reactivity
Human
Cat no : KE00175
Synonyms
Ecalectin, Gal 9, Galectin 9, HUAT, LGALS9, LGALS9A, Tumor antigen HOM HD 21
Validation Data Gallery
Product Information
KE00175 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The LGALS9/Galectin-9 ELISA kit is to be used to detect and quantify protein levels of endogenous LGALS9/Galectin-9. The assay recognizes human LGALS9/Galectin-9. An antibody specific for LGALS9/Galectin-9 has been pre-coated onto the microwells. The LGALS9/Galectin-9 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for LGALS9/Galectin-9 is added to detect the captured LGALS9/Galectin-9 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human LGALS9/Galectin-9 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum,Plasma, Human milk, Urine |
| Assay type | Sandwich |
| Sensitivity | 3.9 pg/mL |
| Range | 31.25-2000 pg/mL, 62.5-4000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3965 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human milk | 94% | 74%-124% |
| Urine | 95% | 73%-108% |
| Serum | 96% | 83%-108% |
| Plasma | 96% | 78%-127% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 105.2 | 2.9 | 2.8 |
| 2 | 20 | 258.1 | 10.9 | 4.2 |
| 3 | 20 | 887.9 | 73.7 | 8.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 113.3 | 4.6 | 4.1 |
| 2 | 24 | 291.9 | 12.9 | 4.4 |
| 3 | 24 | 1,078.3 | 64.0 | 5.9 |
Background Information
The galectins, formerly known as S-type lectins, are a family of β-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-9 (LGALS9) is a tandem repeat-type member of the galectin family. It has three isoforms (named galectin-9L, galectin-9M, and galectin-9S): long type of 355 amino acids, medium type of 323 amino acids, and short type of 311 amino acids. Galectin-9 is ubiquitously expressed in a variety of tissues, including lymph nodes and spleen, and overexpressed in Hodgkin disease tissue. It is involved in chemoattraction, apoptosis, and the regulation of cell differentiation and has anti-allergic effects. It has been reported that galectin-9 is a ligand for Tim-3, through which galectin-9 can induce T-helper type 1 lymphocyte (Th1) death.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Ecalectin, Gal 9, Galectin 9, HUAT, LGALS9, LGALS9A, Tumor antigen HOM HD 21 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Front Immunol Exploring immunological alterations of B cells in peripheral immunity via single-cell RNA sequencing: insights into primary membranous nephropathy. | ||
Hum Immunol The elevation of plasma galectin-9 levels in patients with psoriasis and its associations with inflammatory and immune checkpoint molecules in skin tissues | ||
FASEB J Novel inflammatory markers in intracerebral hemorrhage: Results from Olink proteomics analysis |