Recombinant Human LHB protein (mFc Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

mFc Tag

Activity

not tested

Cat no : Eg4252

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Validation Data Gallery

• 

  Purity of Recombinant Human LHB was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human LHB protein Ser21-Leu141 (Accession# P01229) with a mouse IgG Fc tag at the C-terminus.
• GeneID: 3972
• Accession: P01229
• PredictedSize: 39.8 kDa
• SDS-PAGE: 43-48 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Lutropin Subunit Beta, also known as LHB, is a member of the glycoprotein hormone beta chain family. It encodes the beta subunit of luteinizing hormone (LH), expressed in the pituitary gland, and promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids. LHB is synthesized and secreted by the gonadotropes within the anterior pituitary gland. Mutations in the LHB gene are associated with hypogonadism, characterized by infertility and pseudohermaphroditism.

References:

1.Keutmann HT. et al. (1987). Proc Natl Acad Sci U S A. 84(7):2038-2042.
2.Jablonka-Shariff A. et al. (2008). J Biol Chem. 283(17):11485-11492. 
Lofrano-Porto A. et al. (2007). N Engl J Med. 357(9):897-904.