Human MMP-13 ELISA Kit
Cat no : KE00078
Synonyms
CLG3, Collagenase 3, Matrix metalloproteinase 13, MMP 13, MMP13, MMP-13
Validation Data Gallery
Product Information
KE00078 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The MMP-13 ELISA kit is to be used to detect and quantify protein levels of endogenous MMP-13. The assay recognizes human MMP-13. An antibody specific for MMP-13 has been pre-coated onto the microwells. The MMP-13 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for MMP-13 is added to detect the captured MMP-13 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human MMP-13 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 1.2 pg/mL |
| Range | 93.8-6000 pg/ml |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 4322 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 90% | 82%-100% |
| Cell culture supernatants | 86% | 79%-92% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 928.6 | 69.5 | 7.5 |
| 2 | 20 | 1,630.0 | 101.7 | 6.2 |
| 3 | 20 | 3,808.3 | 227.1 | 6.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 1,002.6 | 78.1 | 7.8 |
| 2 | 24 | 1,833.0 | 111.3 | 6.1 |
| 3 | 24 | 4,422.9 | 363.0 | 8.2 |
Background Information
MMP-13 (Matrix metalloproteinase 13), also known as collagenase-3, cleaves type I collagen and was previously thought to be the rodent analogue of human interstitial collagenase (MMP-1). MMP13 is considered to play a role in the tumor cell proliferation and invasion. MMP13 is a potential prognostic marker for colorectal cancer, breast cancer and other diseases (PMID: 20878116, PMID: 19787229).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CLG3, Collagenase 3, Matrix metalloproteinase 13, MMP 13, MMP13, MMP-13 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Cancer Lett PLD1 promotes tumor invasion by regulation of MMP-13 expression via NF-κB signaling in bladder cancer. | ||
J Clin Med Zinc Administration and Improved Serum Markers of Hepatic Fibrosis in Patients with Autoimmune Hepatitis. | ||
Int J Mol Sci Exosomes Derived from Hypoxia-Cultured Human Adipose Stem Cells Alleviate Articular Chondrocyte Inflammaging and Post-Traumatic Osteoarthritis Progression | ||
Chin Med J (Engl) Development of a novel HER2-CAR monocyte cell therapy with controllable proliferation and enhanced anti-tumor efficacy | ||
Mol Carcinog Breast cancer malignancy is governed by regulation of the macroH2A2/TM4SF1 axis, the AKT/NF-κB pathway, and elevated MMP13 expression | ||
Cartilage miR-708-5p Attenuates Osteoarthritis Progression via Multi-Target Modulation of the NOX4/NF-κB Axis and Cartilage Homeostasis |