Human Total MMP-7 ELISA Kit
Cat no : KE00219
Synonyms
Matrilysin, Matrin, Matrix metalloproteinase 7, MMP 7, MMP7, MMP-7, MPSL1, PUMP 1, Pump 1 protease, PUMP1, Uterine metalloproteinase
Validation Data Gallery
Product Information
KE00219 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Total MMP-7 ELISA kit is to be used to detect and quantify protein levels of endogenous Total MMP-7. The assay recognizes human Total MMP-7. An antibody specific for Total MMP-7 has been pre-coated onto the microwells. The Total MMP-7 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human Total MMP-7 is added to detect the captured human Total MMP-7 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Total MMP-7 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Saliva, Urine |
| Assay type | Sandwich |
| Sensitivity | 3.0 pg/mL |
| Range | 62.5-4000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 4316 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 91% | 75%-119% |
| Human saliva | 102% | 75%-125% |
| Human urine | 101% | 78%-129% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 1,731.7 | 107.1 | 6.2 |
| 2 | 20 | 380.7 | 12.9 | 3.4 |
| 3 | 20 | 100.5 | 6.9 | 6.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 1,921.6 | 206.0 | 10.7 |
| 2 | 24 | 432.9 | 36.0 | 8.5 |
| 3 | 24 | 104.8 | 9.8 | 9.3 |
Background Information
Matrix metalloproteinase-7 (MMP-7)/ matrilysin is a member of the MMP family, but is structurally different from the other MMPs by virtue of the absence of a conserved COOH-terminal protein domain. MMPs are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and cancer metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. MMP-7 degrades proteoglycans, fibronectin, elastin and casein, and is involved in wound healing, tumor progression, pulmonary fibrosis, and development of choroidal neovascularization in age-related macular degeneration. The expression of MMP-7 is increased in most tumors.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Matrilysin, Matrin, Matrix metalloproteinase 7, MMP 7, MMP7, MMP-7, MPSL1, PUMP 1, Pump 1 protease, PUMP1, Uterine metalloproteinase |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.