Human MPO ELISA Kit
Cat no : KE00171
Synonyms
EC:1.11.2.2, myeloperoxidase
Validation Data Gallery
Product Information
KE00171 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The MPO ELISA kit is to be used to detect and quantify protein levels of endogenous MPO. The assay recognizes Human MPO. An antibody specific for MPO has been pre-coated onto the microwells. The MPO protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for MPO is added to detect the captured MPO protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human MPO ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Cell lysates, Saliva |
| Assay type | Sandwich |
| Sensitivity | 9.1 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 4353 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 101% | 80%-126% |
| Cell culture supernatants | 97% | 81%-125% |
| Cell lysates | 105% | 91%-125% |
| saliva | 96% | 87%-110% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 974.4 | 19.5 | 2.0 |
| 2 | 20 | 239.6 | 6.8 | 2.8 |
| 3 | 20 | 50.9 | 4.0 | 7.8 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 961.5 | 36.4 | 3.8 |
| 2 | 24 | 242.7 | 12.7 | 5.2 |
| 3 | 24 | 53.4 | 4.4 | 8.3 |
Background Information
MPO(myeloperoxidase) is a peroxidase enzyme presented in the azurophilic granules of polymorphonuclear (PMN) leukocytes and monocytes. Plasma concentration of MPO can be used as a specific marker of PMN activation. MPO catalyzes the production of hypochlorous acid (HClO) from hydrogen peroxide (H2O2) and chloride anion (Cl-, or the equivalent from a non-chlorine halide). This enzymatic system plays an important role in human defense against microorganisms. The serum/plasma MPO levels have been associated with a variety of clinical conditions including inflammatory diseases, atherosclerosis, ischaemic stroke, hypertension, heart failure, risk of cardiovascular events and so on.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | EC:1.11.2.2, myeloperoxidase |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
PLoS One miR-328-3p targets TLR2 to ameliorate oxygen-glucose deprivation injury and neutrophil extracellular trap formation in HUVECs via inhibition of the NF-κB signaling pathway | ||
J Dermatol A homozygous loss-of-function variant in the MPO gene is associated with generalized pustular psoriasis | ||
Sci Rep Preliminary evidence of immune infiltration and neutrophil degranulation in peripheral blood of non-obese OSA patients related to cognitive decline |