Human NOX4 ELISA Kit
Cat no : KE01022
Synonyms
EC:1.6.3.1, Kidney oxidase 1, Kidney oxidase-1, Kidney superoxide-producing NADPH oxidase, KOX
Validation Data Gallery
Product Information
KE01022 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human NOX4 ELISA kit is to be used to detect and quantify protein levels of endogenous human NOX4. The assay recognizes human NOX4. An antibody specific for human NOX4 has been pre-coated onto the microwells. The human NOX4 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human NOX4 is added to detect the captured human NOX4 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human NOX4 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 0.55 ng/mL |
| Range | 1.25-80 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 50507 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 87% | 82%-96% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 37.94 | 2.77 | 7.30 |
| 2 | 8 | 10.15 | 0.74 | 7.28 |
| 3 | 8 | 5.62 | 0.22 | 3.84 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 39.21 | 2.60 | 6.63 |
| 2 | 16 | 10.43 | 0.85 | 8.17 |
| 3 | 16 | 5.65 | 0.84 | 14.80 |
Background Information
NOX4 (NADPH oxidase 4) is a phagocyte-type oxidase, similar to that responsible for the production of large amounts of reactive oxygen species (ROS) in neutrophil granulocytes with resultant antimicrobial activity and it has been postulated to function in the kidney as an oxygen sensor that regulates the synthesis of erythropoietin in the renal cortex. The truncated NOX4 splice variant D lacks the majority of the transmembrane domain and has been shown to produce higher levels of ROS and DNA damage compared to its prototype. NOX4D has previously been shown to localise to the nucleus and nucleolus in various cell types and is implicated in the generation of reactive oxygen species (ROS) and DNA damage.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | EC:1.6.3.1, Kidney oxidase 1, Kidney oxidase-1, Kidney superoxide-producing NADPH oxidase, KOX |