Human ORM1 ELISA Kit
Cat no : KE00066
Synonyms
AGP 1, AGP A, AGP1, Alpha 1 acid glycoprotein 1, OMD 1, ORM, ORM1, orosomucoid 1
Validation Data Gallery
Product Information
KE00066 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The ORM1 ELISA kit is to be used to detect and quantify protein levels of endogenous ORM1. The assay recognizes human ORM1. An antibody specific for ORM1 has been pre-coated onto the microwells. The ORM1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for ORM1 is added to detect the captured ORM1 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.
| Product name | Human ORM1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Urine |
| Assay type | Sandwich |
| Sensitivity | 0.2 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 5004 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Urine | 79% | 71%-94% |
| Human plasma | 96% | 82%-114% |
| Cell culture supernatants | 96% | 89%-111% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 147.4 | 8.9 | 6.0 |
| 2 | 20 | 431.6 | 15.3 | 3.5 |
| 3 | 20 | 1,500.4 | 51.8 | 3.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 145.5 | 11.5 | 7.9 |
| 2 | 24 | 453.6 | 31.0 | 6.8 |
| 3 | 24 | 1,536.1 | 78.7 | 5.1 |
Background Information
ORM1 (Orosomucoid 1), also named AGP1 (Alpha-1-acid glycoprotein 1) and AGP A, is an abundant plasma protein characterized by anti-inflammatory and immune-modulating properties. In human, two isoforms have been described, named ORM1 and ORM2. ORM1 is implicated in two main different functions: (1) as a lipocalin family member, it exerts a carrier activity especially for drug and lipid molecules; (2) being an acute phase protein (PMID: 23973664). ORM1 are increased in the patients with cancers, burnt injury or surgical operation (PMID: 10913518; PMID: 1646598). ORM1 could be a potential novel urinary biomarker for the early detection of chronic heart failure (PMID: 25215505).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | AGP 1, AGP A, AGP1, Alpha 1 acid glycoprotein 1, OMD 1, ORM, ORM1, orosomucoid 1 |