Human OX40L/TNFSF4 ELISA Kit
Cat no : KE00376
Synonyms
OX40L,TNFSF4,TNFSF4/CD252,OX 40L,OX40 ligand
Validation Data Gallery
Product Information
KE00376 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human OX40L/TNFSF4 ELISA kit is to be used to detect and quantify protein levels of endogenous human OX40L/TNFSF4. The assay recognizes human OX40L/TNFSF4. An antibody specific for human OX40L/TNFSF4 has been pre-coated onto the microwells. The human OX40L/TNFSF4 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human OX40L/TNFSF4 is added to detect the captured human OX40L/TNFSF4 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human OX40L/TNFSF4 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 6.0 pg/mL |
| Range | 46.88-3000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 7292 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 93% | 78%-106% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 1,515.1 | 146.7 | 9.7 |
| 2 | 20 | 399.7 | 33.8 | 8.5 |
| 3 | 20 | 83.8 | 7.5 | 8.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 1,723.0 | 168.9 | 9.8 |
| 2 | 24 | 432.2 | 34.6 | 8.0 |
| 3 | 24 | 85.7 | 8.3 | 9.7 |
Background Information
The OX40 ligand (OX40L) is also named as TNFSF4, CD252, Glycoprotein Gp34 and TXGP1. It belongs to the tumor necrosis factor family. OX40L is a costimulatory molecules that is mainly expressed on APCs, including mature DCs, B cells, and macrophages. OX40L interacts with OX40 (also CD134) that is preferentially expressed on activated CD4 T cells. OX40L expression in DCs is up-regulated by several stimulatory signalings, including thymic stromal lymphopoietin (TSLP), CD40, PAMPs, and IFN-α. Of note, IgE-Ag complex-activated mast cells up-regulate the expression of OX40L, and interactions between OX40L on mast cells and OX40 on T cells is required for T cell proliferation.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | OX40L,TNFSF4,TNFSF4/CD252,OX 40L,OX40 ligand |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.