Speedy™ Human OX40L/TNFSF4 One-Step ELISA Kit
Cat no : SE50004
Synonyms
B2M, Beta-2-microglobulin, OX40L, TNFSF4, TNFSF4/CD252
Validation Data Gallery
Product Information
| Product name | Speedy™ Human OX40L/TNFSF4 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 0.3 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 7292 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 81% | 70%-93% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 409.8 | 12.4 | 3.0 |
| 2 | 8 | 113.5 | 4.8 | 4.2 |
| 3 | 8 | 56.0 | 2.4 | 4.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 423.3 | 21.1 | 5.0 |
| 2 | 16 | 113.9 | 4.3 | 3.8 |
| 3 | 16 | 57.0 | 2.3 | 4.1 |
Background Information
Tumor necrosis factor ligand superfamily member 4 (TNFSF4) is also named as OX40 ligand (OX40L), TXGP1 and CD252. TNFSF4 (OX40L or CD134L) is the only known ligand of TNFRSF4 (OX40 or CD134). It is a type II transmembrane protein that contains the conserved tumor necrosis factor (TNF) homology domain that enables trimerization. TNFSF4 is a co-stimulatory checkpoint protein expressed by several types of immune and non-immune cells, and was shown in the past to enhance the anti-neoplastic activity of T cells. TNFSF4 has long been known to be expressed on antigen presenting cells and to be inducible in T cells . Expression of TNFSF4 by monocytes was shown to promote T follicular helper cell polarization and pathogenesis in human lupus.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | B2M, Beta-2-microglobulin, OX40L, TNFSF4, TNFSF4/CD252 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
2、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data?
Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.