Recombinant Human SELPLG/CD162 protein (rFc Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg1692

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Validation Data Gallery

• 

  Purity of Recombinant Human SELPLG was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human SELPLG protein Gln42-Cys320 (Accession# Q14242-1) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 6404
• Accession: Q14242-1
• PredictedSize: 55.1 kDa
• SDS-PAGE: 80-90 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

SELPLG (selectin P ligand), also known as CD162 or PSGL-1, is a type I transmembrane mucin-like glycoprotein. It is an adhesion molecule expressed on the surface of neutrophils, monocytes, and most lymphocytes as a homodimer of approximately 230-250 kDa (PMID: 7545173; 1378449; 9353350). SELPLG plays a critical role in the early stages of inflammation due to its ability to bind E-selectin (CD62E), L-selectin (CD62L), and P-selectin (CD62P) (PMID: 35597982).

References:

1. Vachino G. et al. (1995). J Biol Chem. 270(37):21966-21974.
2. Moore KL. et al. (1992). J Cell Biol. 118(2):445-456.
3. Borges E. et al. (1997). J Biol Chem. 272(45):28786-28792.
4. Burnie J. et al. (2022). Retrovirology. 19(1):9.