Human Prolactin ELISA Kit
Cat no : KE00172
Synonyms
PRL, prolactin
Validation Data Gallery
Product Information
KE00172 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The PRL ELISA kit is to be used to detect and quantify protein levels of endogenous PRL. The assay recognizes human PRL. An antibody specific for PRL has been pre-coated onto the microwells. The PRL protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for PRL is added to detect the captured PRL protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Prolactin ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Human milk |
| Assay type | Sandwich |
| Sensitivity | 1.4 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 5617 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| human serum | 99% | 89%-110% |
| Cell culture supernatants | 94% | 78%-109% |
| human milk | 103% | 89%-123% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 101.7 | 8.5 | 8.4 |
| 2 | 20 | 305.6 | 5.6 | 1.8 |
| 3 | 20 | 1,054.2 | 21.8 | 2.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 72.9 | 4.1 | 5.1 |
| 2 | 24 | 264.1 | 7.1 | 2.7 |
| 3 | 24 | 958.0 | 28.2 | 2.9 |
Background Information
Prolactin (PRL) is a pleiotropic hormone which is mainly synthetized by the lactotrophs of the anterior pituitary gland. Prolactin has a wide variety of effects. It causes the breasts to grow and make milk during pregnancy and after birth. Prolactin levels are normally high for pregnant women and new mothers. Prolactin also plays an essential role in metabolism, regulation of the immune system and pancreatic development. Normally during pregnancy prolactin serum levels are increased to hold of insulin resistance (IR). As well, prolactin is involved in regulation of glucose metabolism, since high prolactin levels are associated with low incidence of type 2 diabetes mellitus (T2DM).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | PRL, prolactin |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Reprod Sci A Novel Method to Repair Thin Endometrium and Restore Fertility Based on Menstruation-Derived Stem Cell | ||
Jpn J Nurs Sci A program for accelerating lactogenesis II using a breast pump shortly after cesarean section in Japan: A feasibility mixed-methods study. |