Human Resistin ELISA Kit
Cat no : KE00159
Synonyms
ADSF, FIZZ3, HXCP1, resistin, RETN, RETN1, RSTN, XCP1
Validation Data Gallery
Product Information
KE00159 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Resistin ELISA kit is to be used to detect and quantify protein levels of endogenous Resistin. The assay recognizes human Resistin. An antibody specific for Resistin has been pre-coated onto the microwells. The Resistin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for Resistin is added to detect the captured Resistin protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Resistin ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 15.2 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 56729 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human Plasma | 89% | 79%-103% |
| Cell culture supernatants | 86% | 77%-96% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 977.2 | 35.8 | 3.7 |
| 2 | 20 | 216.5 | 10.9 | 5.0 |
| 3 | 20 | 51.5 | 3.7 | 7.2 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 961.4 | 47.4 | 4.9 |
| 2 | 24 | 224.2 | 10.5 | 4.7 |
| 3 | 24 | 52.3 | 3.3 | 6.2 |
Background Information
Resistin, a cysteine-rich hormone secreted primarily by rodent fat cells, was postulated to be implicated in obesity, insulin resistance and diabetes. Resistin is also secreted by macrophages in humans, promoting inflammation (PMID: 20148674). Resistin modulates the synthesis and secretion of key pro-inflammatory cytokines such as TNF-α, IL-6 and IL12 in macrophages via a nuclear factor-kB (NF-kB)-dependent pathway. Given the connection between inflammation and cancer, resistin was soon considered for its role in cancer. There were observations supporting its possible use as a diagnostic marker, expression levels of which also seemed to differ in patients with varying clinical stages (PMID: 30317640). Resistin has been also linked to other pathologies such as atherosclerosis (PMID: 15710760).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | ADSF, FIZZ3, HXCP1, resistin, RETN, RETN1, RSTN, XCP1 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.