Human S100A8/S100A9 ELISA Kit
Cat no : KE00177
Synonyms
60B8AG, CAGA, Calgranulin A, Calprotectin L1L subunit, CFAG, CGLA, CP 10, Cystic fibrosis antigen, L1Ag, MA387, MIF, MRP 8, MRP8, NIF, P8, Protein S100 A8, S100A8, Urinary stone protein band A
Validation Data Gallery
Product Information
KE00177 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The S100A8/S100A9 ELISA kit is to be used to detect and quantify protein levels of endogenous S100A8/S100A9. The assay recognizes human S100A8/S100A9. An antibody specific for S100A8/S100A9 has been pre-coated onto the microwells. The S100A8/S100A9 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human S100A8/S100A9 is added to detect the captured human S100A8/S100A9 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm .
| Product name | Human S100A8/S100A9 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Cell culture supernatants, Urine, Saliva, Human milk |
| Assay type | Sandwich |
| Sensitivity | 11.8 pg/mL |
| Range | 62.5-4000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 6279 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 92% | 79%- 123% |
| Cell culture supernatants | 92% | 81%- 106% |
| Human milk | 89% | 76%- 122% |
| Urine | 80% | 76%- 96% |
| Saliva | 93% | 78%- 119% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 1,816.5 | 76.8 | 4.2 |
| 2 | 20 | 413.2 | 18.6 | 4.5 |
| 3 | 20 | 92.4 | 6.1 | 6.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 1,994.1 | 126.3 | 6.3 |
| 2 | 24 | 457.5 | 38.9 | 8.5 |
| 3 | 24 | 115.4 | 11.1 | 9.7 |
Background Information
S100A8 and S100A9 (also known as MRP8 and MRP14, respectively) are Ca2+ binding proteins belonging to the S100 family. Calprotectin, is a heterodimeric calcium-binding protein consisting of S100A8 and S100A9 subunits. They are expressed in a wide variety of cell types and are abundant in myeloid cells such as: neutrophils, monocytes, keratinocytes and early differentiation states of macrophages. S100A8 and S100A9 have already been confirmed to play a decisive role in the development of inflammation. Elevated levels of S100A8/S100A9 were detected in inflammation, neoplastic tumor cells and various human cancers.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | 60B8AG, CAGA, Calgranulin A, Calprotectin L1L subunit, CFAG, CGLA, CP 10, Cystic fibrosis antigen, L1Ag, MA387, MIF, MRP 8, MRP8, NIF, P8, Protein S100 A8, S100A8, Urinary stone protein band A |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Front Mol Biosci Expression and clinical significance of S100A8/9 in adults with secondary phagocytic lymphohistiocytosis | ||
Cell Death Dis Gasdermin E promotes translocation of p65 and c-jun into nucleus in keratinocytes for progression of psoriatic skin inflammation | ||
J Exp Clin Cancer Res S100A9+CD14+ monocytes contribute to anti-PD-1 immunotherapy resistance in advanced hepatocellular carcinoma by attenuating T cell-mediated antitumor function | ||
Respir Res Extracellular vesicle-bound S100A8/A9 is differentially expressed in septic shock and prompts acute lung injury | ||
Oncogene Chronic stress drives gastric cancer lymph node metastasis via NETosis through a feedforward ACh-NGF axis-mediated PKC-γ/STAT3 pathway. | ||
Cell Rep Med Alarmin S100A8 imparts chemoresistance of esophageal cancer by reprogramming cancer-associated fibroblasts |