SARS-CoV-2 N protein SARS-CoV-2 N protein ELISA Kit
Sensitivity
38 pg/mL
Range
375-6000 pg/mL
Reactivity
SARS-CoV-2 N protein
Cat no : KE30007
Synonyms
nucleocapsid phosphoprotein, SARS-COV-2 nucleocapsid phosphoprotein, N protein, sars cov 2
Validation Data Gallery
Product Information
KE30007 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The SARS-CoV-2 N protein ELISA kit is to be used to detect and quantify protein levels of endogenous SARS-CoV-2 N protein. The assay recognizes human SARS-CoV-2 N protein. An antibody specific for SARS-CoV-2 N protein has been pre-coated onto the microwells. The SARS-CoV-2 N protein in samples is captured by the coated antibody after incubation, Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for SARS-CoV-2 N is added to detect the captured SARS-CoV-2 N protein. followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | SARS-CoV-2 N protein SARS-CoV-2 N protein ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Human plasma |
| Assay type | Sandwich |
| Sensitivity | 38 pg/mL |
| Range | 375-6000 pg/mL |
| Reactivity | SARS-CoV-2 N protein |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 43740575 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 116% | 95%-127% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 3011.9 | 119.3 | 4.0 |
| 2 | 20 | 693.7 | 38.5 | 5.5 |
| 3 | 20 | 327.1 | 22.5 | 6.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 2936.3 | 76.7 | 2.6 |
| 2 | 24 | 682.9 | 30.1 | 4.4 |
| 3 | 24 | 326.6 | 22.9 | 7.0 |
Background Information
Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | nucleocapsid phosphoprotein, SARS-COV-2 nucleocapsid phosphoprotein, N protein, sars cov 2 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
ACS Appl Nano Mater Lethal Interactions of SARS-CoV-2 with Graphene Oxide: Implications for COVID-19 Treatment | ||
BMJ Open Determining the incidence, risk factors and biological drivers of irritable bowel syndrome (IBS) as part of the constellation of postacute sequelae of SARS-CoV-2 infection (PASC) outcomes in the Arizona CoVHORT-GI: a longitudinal cohort study | ||