Human TDP-43 ELISA Kit

Sensitivity

5.0 pg/mL

Range

78.1-5000 pg/mL

Reactivity

Human

Publications

3

Cat no : KE00005

Synonyms

ALS10, TAR DNA binding protein, TAR DNA binding protein 43, TARDBP, TDP 43, TDP43, TDP-43

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Validation Data Gallery

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  Standard curve of KE00005 Human TDP-43 ELISA Kit.

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Product Information

KE00005 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The TDP-43 ELISA kit is to be used to detect and quantify protein levels of endogenous TDP-43. The assay recognizes human TDP-43. An antibody specific for TDP-43 has been pre-coated onto the microwells. The TDP-43 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for TDP-43 is added to detect the captured TDP-43 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

• Product name: Human TDP-43 ELISA Kit
• Tests: 1 X 96 well plate
• Sample type: Serum, Plasma
• Assay type: Sandwich
• Sensitivity: 5.0 pg/mL
• Range: 78.1-5000 pg/mL
• Reactivity: Human
• Tested applications: Sandwich ELISA

Recovery

Sample Type	Average	Range
Human plasma	88%	75%-109%

IntraAssay

Sample	n	mean ( pg/mL)	SD	CV%
1	20	2,335.4	52.9	2.3
2	20	623.9	19.3	3.1
3	20	269.9	13.2	4.9

InterAssay

Sample	n	mean ( pg/mL)	SD	CV%
1	24	2,178.4	171.5	7.9
2	24	550.5	52.8	9.6
3	24	265.7	13.1	4.9

Background Information

TARDBP was firstly found as a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. It was also reported to regulate alternate splicing of the CFTR gene. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP43, is the major disease protein in ubiquitin-positive, tau-, and alpha-synuclein-negative frontotemporal dementia (FTLD-U) and in Amyotrophic lateral sclerosis (ALS). KE00005 is used to quantify TDP-43 level in vivo and it is suitable for sample of serum or plasma. KE00009 is used to quantify TDP-43 level in vivo and it is suitable for sample of CSF and cell lysates.

Properties

Storage Instructions	All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms	ALS10, TAR DNA binding protein, TAR DNA binding protein 43, TARDBP, TDP 43, TDP43, TDP-43

Publications

Species	Sample Type	Title
human		Neurochem Int; TDP-43 levels are higher in platelets from patients with sporadic amyotrophic lateral sclerosis than in healthy controls.; Authors - Miki Hishizawa
human	plasma,cerebrospinal fluid	Front Neurol; TDP-43 and Phosphorylated TDP-43 Levels in Paired Plasma and CSF Samples in Amyotrophic Lateral Sclerosis.; Authors - Yuting Ren
human	plasma	Neurol Neuroimmunol Neuroinflamm; TDP-43-specific Autoantibody Decline in Patients With Amyotrophic Lateral Sclerosis.; Authors - Anne Kallehauge Nielsen