Human TFPI ELISA Kit
Cat no : KE00202
Synonyms
EPI, Extrinsic pathway inhibitor, LACI, TFI, TFPI, TFPI1
Validation Data Gallery
Product Information
KE00202 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The TFPI ELISA kit is to be used to detect and quantify protein levels of endogenous TFPI. The assay recognizes human TFPI. An antibody specific for TFPI has been pre-coated onto the microwells. The TFPI protein in samples is captured by the coated antibody after incubation.Following extensive washing, another antibody of biotinylated specific for TFPI is added to detect the captured TFPI protein. For signal development, Sterptravidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human TFPI ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 7.2 pg/mL |
| Range | 15.6-1000 pg/mL , 62.5-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 7035 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 75%-122% | 107% |
| Cell culture supernatants | 72%-120% | 89% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 23.3 | 2.2 | 9.4 |
| 2 | 20 | 75.7 | 5.4 | 7.1 |
| 3 | 20 | 312.9 | 15.6 | 5.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 22.9 | 1.5 | 6.6 |
| 2 | 24 | 66.8 | 5.6 | 8.5 |
| 3 | 24 | 314.2 | 18.1 | 5.8 |
Background Information
Tissue factor pathway inhibitor (TFPI) is a critical anticoagulant protein present in endothelium and platelets. TFPI is produced as two major isoforms in humans, TFPIa and TFPIb that result from alternative splicing. The TFPIb isoform localizes to the endothelium surface where it is a potent inhibitor of tissue factor-factor VIIa complexes that initiate blood coagulation. The TFPIa isoform is present in platelets. TFPI is a Kunitz-type serine protease inhibitor that exerts anticoagulant activity by blocking early procoagulant stimulia. TFPI can enhance anti-thrombotic treatment in sepsis, inflammatory diseases, and cardiovascular diseases. In plasma, 80% of TFPI is carboxy-terminal truncated and circulates bound primarily to low-density lipoproteins (LDL), with the levels of the latter having an impact on plasma TFPI levels. The other 20% is unbound free-form TFPI characterised by the K3 domain.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | EPI, Extrinsic pathway inhibitor, LACI, TFI, TFPI, TFPI1 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.