Human TNFRSF6B/DcR3 ELISA Kit
Cat no : KE00188
Synonyms
DCR3, Decoy receptor 3, Decoy receptor for Fas ligand, DJ583P15.1.1, M68, TNFRSF6B, TR6
Validation Data Gallery
Product Information
KE00188 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The TNFRSF6B/DcR3 ELISA kit is to be used to detect and quantify protein levels of endogenous TNFRSF6B/DcR3. The assay recognizes human TNFRSF6B/DcR3. An antibody specific for TNFRSF6B/DcR3 has been pre-coated onto the microwells. The TNFRSF6B/DcR3 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human TNFRSF6B/DcR3 is added to detect the captured human TNFRSF6B/DcR3 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human TNFRSF6B/DcR3 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysates |
| Assay type | Sandwich |
| Sensitivity | 3.4 pg/mL |
| Range | 39-2500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 8771 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysates | 84% | 75%-96% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 1,145.1 | 27.5 | 2.4 |
| 2 | 20 | 264.4 | 5.0 | 1.9 |
| 3 | 20 | 62.8 | 2.3 | 3.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 1,200.1 | 24.8 | 2.1 |
| 2 | 24 | 262.6 | 6.1 | 2.3 |
| 3 | 24 | 55.6 | 2.3 | 4.2 |
Background Information
Tumor necrosis factor receptor superfamily member 6B (TNFRSF6B), also identified as DcR3 or M68, is a secreted member of the Tumor Necrosis Factor (TNF) receptor superfamily, neutralizes three different TNF ligands: TNFS14/LIGHT, TNFSF15/TL1A and TNFSF6/FasL. TNFRSF6B binds to FasL and inhibits FasL-induced apoptosis. It can also bind to LIGHT and suppresse LIGHT-mediated apoptosis. Interaction of TL1A with DR3 promotes T cell expansion during an immune response. Expression of DcR3 is significantly elevated in patients with a range of cancers and autoimmune diseases.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | DCR3, Decoy receptor 3, Decoy receptor for Fas ligand, DJ583P15.1.1, M68, TNFRSF6B, TR6 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.