Human Thrombospondin 1 ELISA Kit
Cat no : KE00249
Synonyms
THBS, THBS 1, THBS1, thrombospondin 1, Thrombospondin-1, TSP, TSP 1, TSP1, TSP-1
Validation Data Gallery
Product Information
KE00249 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Thrombospondin 1 ELISA kit is to be used to detect and quantify protein levels of endogenous Thrombospondin 1 . The assay recognizes human Thrombospondin 1 . An antibody specific for Thrombospondin 1 has been pre-coated onto the microwells. The Thrombospondin 1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human Thrombospondin 1 is added to detect the captured human Thrombospondin 1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Thrombospondin 1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Saliva, Human milk |
| Assay type | Sandwich |
| Sensitivity | 0.002 ng/mL |
| Range | 0.137-100 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 7057 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 81% | 70%-101% |
| Cell culture supernatants | 88% | 72%-100% |
| Saliva | 84% | 70%-117% |
| Human milk | 88% | 77%-114% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 44.43 | 4.17 | 9.4 |
| 2 | 20 | 3.71 | 0.31 | 8.3 |
| 3 | 20 | 0.31 | 0.03 | 9.6 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 36.57 | 3.67 | 10.0 |
| 2 | 24 | 3.66 | 0.20 | 5.5 |
| 3 | 24 | 0.34 | 0.03 | 8.3 |
Background Information
Thrombospondin 1(TSP-1), an extracellular matrix protein, is the first identified natural angiogenesis inhibitor. A variety of normal cells, including endothelial cells, fibroblasts, adipocytes, smooth muscle cells, monocytes, macrophages, and transformed cells such as malignant glioma cells, secrete Thrombospondin 1. It regulates cellular phenotype during tissue genesis and repair. It acts as a molecular facilitator by bringing together cytokines, growth factors, matrix components, membrane receptors and extracellular proteases. Thrombospondin 1 binds to a wide variety of integrin and non-integrin cell surface receptors. It is also a major activator of transforming growth factor (TGFβ1).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | THBS, THBS 1, THBS1, thrombospondin 1, Thrombospondin-1, TSP, TSP 1, TSP1, TSP-1 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Redox Biol Endothelial transferrin receptor 1 contributes to thrombogenesis through cascade ferroptosis | ||
Ann Hematol Lycorine inhibits the proliferation of acute myeloid leukemia cells by upregulating the expression of THBS1. |