Human Uteroglobin ELISA Kit
Cat no : KE00099
Synonyms
CC10, CC16, CCPBP, CCSP, SCGB1A1, UGB, UP 1, UP1, Urinary protein 1, Urine protein 1, Uteroglobin
Validation Data Gallery
Product Information
KE00099 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Uteroglobin ELISA kit is to be used to detect and quantify protein levels of endogenous Uteroglobin. The assay recognizes human Uteroglobin. An antibody specific for Uteroglobin has been pre-coated onto the microwells. The Uteroglobin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for Uteroglobin is added to detect the captured Uteroglobin protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Uteroglobin ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Urine |
| Assay type | Sandwich |
| Sensitivity | 14.6 pg/mL |
| Range | 78.1 - 5000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 7356 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Urine | 99% | 83%-119% |
| Human plasma | 100% | 78%-121% |
| Cell culture supernatants | 100% | 80%-120% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 253.0 | 15.03 | 5.9 |
| 2 | 20 | 533.3 | 40.75 | 7.6 |
| 3 | 20 | 2038.0 | 199.07 | 9.8 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 115.9 | 11.13 | 9.6 |
| 2 | 24 | 510.4 | 46.19 | 9.0 |
| 3 | 24 | 2180.9 | 178.03 | 8.2 |
Background Information
Uteroglobin, also named clara cell secretory protein or CC16, is mainly secreted by the club cells along the tracheobronchial tree, diffuses passively into the bloodstream and is rapidly eliminated by glomerular filtration. It is a steroid-dependent, immunomodulatory, cytokine-like protein, and it is secreted by the mucosal epithelial cells of many vertebrates and takes part in some signaling pathways. Growing evidence indicates that Uteroglobin has anti-inflammatory and anti-toxicant properties in the lung and may protect against obstructive lung diseases. Lower levels of Uteroglobin in blood and airways have been associated with prevalence and severity of Chronic obstructive pulmonary disease (COPD). Defects in Uteroglobin are associated with a susceptibility to asthma.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CC10, CC16, CCPBP, CCSP, SCGB1A1, UGB, UP 1, UP1, Urinary protein 1, Urine protein 1, Uteroglobin |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.