Human YBX1 ELISA Kit
Cat no : KE00906
Synonyms
CBF A, CBF-A, CCAAT-binding transcription factor I subunit A, DBPB, DNA-binding protein B
Validation Data Gallery
Product Information
KE00906 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human YBX1 ELISA kit is to be used to detect and quantify protein levels of endogenous human YBX1. The assay recognizes human YBX1. An antibody specific for human YBX1 has been pre-coated onto the microwells. The human YBX1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human YBX1 is added to detect the captured human YBX1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human YBX1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 18.6 pg/mL |
| Range | 39-2500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 4904 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 77% | 75%-80% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,194.1 | 48.8 | 4.1 |
| 2 | 8 | 314.1 | 15.8 | 5.0 |
| 3 | 8 | 157.5 | 5.5 | 3.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,150.2 | 66.4 | 5.8 |
| 2 | 16 | 311.3 | 20.4 | 6.6 |
| 3 | 16 | 155.7 | 7.8 | 5.0 |
Background Information
Y-box binding protein 1 (YBX1), also known as YB-1, is a multifunctional member of the cold shock domain protein family and serves as a significant transcription factor. It regulates transcription by binding to Y-box sequences found in the enhancer and promoter regions of various genes, thereby modulating the expression of numerous genes associated with cellular proliferation, senescence, survival, drug resistance, and chromosomal instability. This regulation implicates YBX1 in a broad spectrum of biological processes, such as apoptosis, cell proliferation, differentiation, and stress response. Additionally, YBX1 is a key member of the RNA-binding protein (RBP) family, playing an important role in regulating RNA modifications and metabolism.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CBF A, CBF-A, CCAAT-binding transcription factor I subunit A, DBPB, DNA-binding protein B |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.