ID1 Monoclonal antibody

ID1 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

CloneNo.

1H3G11

Cat no : 67827-1-Ig

Synonyms

bHLHb24, ID, ID1, Inhibitor of DNA binding 1

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Validation Data Gallery

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  WB analysis using 67827-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67827-1-Ig (ID1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 67827-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67827-1-Ig (ID1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 67827-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67827-1-Ig (ID1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, PC-3 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, 4T1 cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 1 publications below

Product Information

67827-1-Ig targets ID1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ID1 fusion protein Ag13359
• Full Name: inhibitor of DNA binding 1, dominant negative helix-loop-helix protein
• Calculated Molecular Weight: 16 kDa
• Observed Molecular Weight: 18 kDa
• GenBank Accession Number: BC000613
• Gene Symbol: ID1
• Gene ID (NCBI): 3397
• RRID: AB_2918589
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ID (inhibitor of DNA binding) proteins contain a helix-loop-helix (HLH) motif and regulate tissue-specific transcription within several cell lineages. They do not bind DNA directly, but inhibit lineage commitment by binding basic helix-loop-helix (bHLH) transcription factors through their HLH motif. ID1 proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID proteins contribute to cell growth, senescence, differentiation, and angiogenesis. Inhibitor of differentiation or DNA binding-1 (ID1) interacts with the transactivation domain of p65 (p65TAD) both in vitro and in vivo. The molecular weight of ID1 is about 18 kDa.

Publications

Species	Application	Title
human	WB	Oncogenesis; Neuron-specific enolase promotes stem cell-like characteristics of small-cell lung cancer by downregulating NBL1 and activating the BMP2/Smad/ID1 pathway.; Authors - Lin Lu
rat	WB	Front Pharmacol; Novel Pyrazolo[3,4-b] Pyridine Derivative (HLQ2g) Attenuates Hypoxic Pulmonary Hypertension via Restoring cGKI Expression and BMP Signaling Pathway; Authors - Lijun Li
human	WB	Pulm Circ; Antiproliferative effect of selexipag active metabolite MRE-269 on pulmonary arterial smooth muscle cells from patients with chronic thromboembolic pulmonary hypertension; Authors - Kazuya Kuramoto
human	WB,IHC	Int J Biol Sci; Nicotinamide adenine dinucleotide kinase promotes lymph node metastasis of NSCLC via activating ID1 expression through BMP pathway; Authors - Zimei Zeng