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IDH2 Polyclonal antibody
IDH2 Polyclonal Antibody for IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat and More (1)
WB, IP, IHC, IF, ELISA
Cat no : 15932-1-AP
Validation Data Gallery
|Positive WB detected in||HEK-293 cells, mouse skeletal muscle tissue, Jurkat cells, mouse brain tissue, mouse heart tissue, rat liver tissue, SH-SY5Y cells, NIH/3T3 cells, HeLa cells, mouse liver tissue, HepG2 cells, K-562 cells|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human colon cancer tissue, mouse kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 4 publications below|
|WB||See 33 publications below|
|IHC||See 4 publications below|
|IF||See 1 publications below|
|IP||See 1 publications below|
15932-1-AP targets IDH2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, chicken|
|Host / Isotype||Rabbit / IgG|
|Immunogen||IDH2 fusion protein Ag8779|
|Full Name||isocitrate dehydrogenase 2 (NADP+), mitochondrial|
|Calculated molecular weight||452 aa, 51 kDa|
|Observed molecular weight||41-47 kDa|
|GenBank accession number||BC009244|
|Gene ID (NCBI)||3418|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
IDH2, also named as IDP and ICD-M, belongs to the isocitrate and isopropylmalate dehydrogenases family. It plays a role in intermediary metabolism and energy production. IDH2 is a mitochondrial NADP-dependent isocitrate dehydrogenase that catalyzes oxidative decarboxylation of isocitrate to alpha-ketoglutarate, producing NADPH. It may tightly associate or interact with the pyruvate dehydrogenase complex. IDH1 and IDH2 mutations could also contribute to tumorigenesis and cancer progression through increased mutagenesis. IDH2 has 2 isoforms with the MW of 51 kDa and 45 kDa, and the mature form is about 47 kDa and 41 kDa with the N-terminal transit peptide cleaved.
|Product Specific Protocols|
|WB protocol for IDH2 antibody 15932-1-AP||Download protocol|
|IHC protocol for IDH2 antibody 15932-1-AP||Download protocol|
|IP protocol for IDH2 antibody 15932-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2.
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STAT3-dependent analysis reveals PDK4 as independent predictor of recurrence in prostate cancer.
SIRT3-Mediated Dimerization of IDH2 Directs Cancer Cell Metabolism and Tumor Growth.
MUC1 triggers lineage plasticity of Her2 positive mammary tumors
CUE domain-containing protein 2 promotes the Warburg effect and tumorigenesis.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Tanusree (Verified Customer) (12-18-2019)
Product worked well in WB at 1:500 dilution