Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55158-1-AP targets IDUA in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | iduronidase, alpha-L- |
| Calculated Molecular Weight | 73 kDa |
| Observed Molecular Weight | 73 kDa |
| GenBank Accession Number | NM_000203 |
| Gene Symbol | IDUA |
| Gene ID (NCBI) | 3425 |
| RRID | AB_11182946 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P35475 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IDUA belongs to the glycosyl hydrolase 39 family. It hydrolysis of unsulfated alpha-L-iduronosidic linkages in dermatan sulfate. Defects in IDUA are the cause of mucopolysaccharidosis type 1H (MPS1H)( also known as Hurler syndrome), MPS1H/S(also known as Hurler-Scheie syndrome) and MPS1S(also known as Scheie syndrome), which are rare lysosomal storage diseases. This antibody is specific to IDUA.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for IDUA antibody 55158-1-AP | Download protocol |
| WB protocol for IDUA antibody 55158-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The IDUA Polyclonal antibody (Cat# 55158-1-AP) has 5 citations published in Mol Ther, Front Immunol, Mol Ther Methods Clin Dev, Mol Cell Biochem, and Hum Gene Ther. Research fields span liver-directed gene therapy for mucopolysaccharidosis type I-H (Hurler syndrome), renal clear cell carcinoma immunotherapy response prediction, and bone mineral density regulation. Applications cited include WB, IHC, IP, and ELISA in human and mouse species.
| Species | Application | Title |
|---|---|---|
Mol Ther Comparative evaluation of liver-directed knockin strategies with viral and nonviral vectors in mouse inherited disease models. | ||
Front Immunol Leveraging diverse cell-death related signature predicts the prognosis and immunotherapy response in renal clear cell carcinoma | ||
Mol Ther Methods Clin Dev Liver-directed gene therapy corrects neurologic disease in a murine model of mucopolysaccharidosis type I-Hurler. | ||
Mol Cell Biochem Why SNP rs3755955 is associated with human bone mineral density? A molecular and cellular study in bone cells. | ||
Hum Gene Ther Lipid Nanoparticle-Delivered mRNA Therapy Corrects Neonatal Murine MPS I-H. |
