IDUA Polyclonal antibody

IDUA Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 55158-1-AP

Synonyms

Alpha L iduronidase, IDA, IDUA, iduronidase, alpha L, MPS1

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Validation Data Gallery

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  WB analysis using 55158-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55158-1-AP (IDUA antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 55158-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55158-1-AP (IDUA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 55158-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 55158-1-AP (IDUA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 55158-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 55158-1-AP (IDUA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Product Information

55158-1-AP targets IDUA in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: iduronidase, alpha-L-
• Calculated Molecular Weight: 73 kDa
• Observed Molecular Weight: 73 kDa
• GenBank Accession Number: NM_000203
• Gene Symbol: IDUA
• Gene ID (NCBI): 3425
• RRID: AB_11182946
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IDUA belongs to the glycosyl hydrolase 39 family. It hydrolysis of unsulfated alpha-L-iduronosidic linkages in dermatan sulfate. Defects in IDUA are the cause of mucopolysaccharidosis type 1H (MPS1H)( also known as Hurler syndrome), MPS1H/S(also known as Hurler-Scheie syndrome) and MPS1S(also known as Scheie syndrome), which are rare lysosomal storage diseases. This antibody is specific to IDUA.

Publications

Species	Application	Title
human	WB	Mol Ther Methods Clin Dev; Liver-directed gene therapy corrects neurologic disease in a murine model of mucopolysaccharidosis type I-Hurler.; Authors - Xiu Jin
	IP	Mol Cell Biochem; Why SNP rs3755955 is associated with human bone mineral density? A molecular and cellular study in bone cells.; Authors - Pei He
human	WB,IHC	Front Immunol; Leveraging diverse cell-death related signature predicts the prognosis and immunotherapy response in renal clear cell carcinoma; Authors - Zhengqi Wu