|Positive WB detected in||A375 cells, PC-3 cells|
|Positive IP detected in||A375 cells|
|Positive IHC detected in||human skin cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
13378-1-AP targets IFIT5 in WB, IP, IHC,ELISA applications and shows reactivity with human samples.
|Host / Isotype||Rabbit / IgG|
|Immunogen||IFIT5 fusion protein Ag4202|
|Full Name||interferon-induced protein with tetratricopeptide repeats 5|
|Calculated molecular weight||482 aa, 58 kDa|
|Observed molecular weight||56-58 kDa, 48 kDa|
|GenBank accession number||BC025786|
|Gene ID (NCBI)||24138|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
IFIT5, also named as Interferon-induced 58 kDa protein or RI58, is a 482 amino acid protein, which contains eight TPR repeats and belongs to the IFIT family. IFIT5 localizes in the Cell projection. Interferon-induced RNA-binding protein that specifically binds single-stranded RNA bearing a 5'-triphosphate group (PPP-RNA), thereby acting as a sensor of viral single-stranded RNAs.
Am J Clin Exp Urol
Interferon-induced IFIT5 promotes epithelial-to-mesenchymal transition leading to renal cancer invasion.
Cell Death Dis
The roles and mechanism of IFIT5 in bladder cancer epithelial-mesenchymal transition and progression.
Acta Biochim Biophys Sin (Shanghai)
IFIT5 potentiates anti-viral response through enhancing innate immune signaling pathways.