|Positive WB detected in||A375 cells, PC-3 cells|
|Positive IP detected in||A375 cells|
|Positive IHC detected in||human skin cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
13378-1-AP targets IFIT5 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.
|Host / Isotype||Rabbit / IgG|
|Immunogen||IFIT5 fusion protein Ag4202|
|Full Name||interferon-induced protein with tetratricopeptide repeats 5|
|Calculated molecular weight||482 aa, 58 kDa|
|Observed molecular weight||56-58 kDa, 48 kDa|
|GenBank accession number||BC025786|
|Gene ID (NCBI)||24138|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
IFIT5, also named as Interferon-induced 58 kDa protein or RI58, is a 482 amino acid protein, which contains eight TPR repeats and belongs to the IFIT family. IFIT5 localizes in the Cell projection. Interferon-induced RNA-binding protein that specifically binds single-stranded RNA bearing a 5'-triphosphate group (PPP-RNA), thereby acting as a sensor of viral single-stranded RNAs.
Am J Clin Exp Urol
Interferon-induced IFIT5 promotes epithelial-to-mesenchymal transition leading to renal cancer invasion.
Cell Death Dis
The roles and mechanism of IFIT5 in bladder cancer epithelial-mesenchymal transition and progression.
Development and validation of an interferon signature predicting prognosis and treatment response for glioblastoma.
PKR Binds Enterovirus IRESs, Displaces Host Translation Factors, and Impairs Viral Translation to Enable Innate Antiviral Signaling.
Acta Biochim Biophys Sin (Shanghai)
IFIT5 potentiates anti-viral response through enhancing innate immune signaling pathways.
IFIT5 positively regulates NF-κB signaling through synergizing the recruitment of IκB kinase (IKK) to TGF-β-activated kinase 1 (TAK1).