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  • KD/KO Validated

IFIT5 Antibody

IFIT5 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13378-1-AP

Synonyms

IFIT 5, IFIT5, RI58



Tested Applications

Positive WB detected inA375 cells, PC-3 cells
Positive IP detected inA375 cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

13378-1-AP targets IFIT5 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IFIT5 fusion protein Ag4202
Full Name interferon-induced protein with tetratricopeptide repeats 5
Calculated molecular weight 482aa,58 kDa
Observed molecular weight 56-58 kDa,48 kDa
GenBank accession number BC025786
Gene symbol IFIT5
Gene ID (NCBI) 24138
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IFIT5, also named as Interferon-induced 58 kDa protein or RI58, is a 482 amino acid protein, which contains eight TPR repeats and belongs to the IFIT family. IFIT5 localizes in the Cell projection. Interferon-induced RNA-binding protein that specifically binds single-stranded RNA bearing a 5'-triphosphate group (PPP-RNA), thereby acting as a sensor of viral single-stranded RNAs.

Protocols

Product Specific Protocols
WB protocol for IFIT5 antibody 13378-1-APDownload protocol
IHC protocol for IFIT5 antibody 13378-1-APDownload protocol
IP protocol for IFIT5 antibody 13378-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Am J Clin Exp Urol

Interferon-induced IFIT5 promotes epithelial-to-mesenchymal transition leading to renal cancer invasion.

Authors - U-Ging Lo
  • KD Validated
humanWB,IHC

Cell Death Dis

The roles and mechanism of IFIT5 in bladder cancer epithelial-mesenchymal transition and progression.

Authors - Jun Huang
HumanWB

Acta Biochim Biophys Sin (Shanghai)

IFIT5 potentiates anti-viral response through enhancing innate immune signaling pathways.

Authors - Zhang Bianhong B