IFITM2/3 Monoclonal antibody

• Featured Product
• KD/KO Validated

IFITM2/3 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

12

CloneNo.

2E8D12

Reviews (1)

Cat no : 66081-1-Ig

Synonyms

1 8U, IFITM2/3, IFITM3, IP15

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Validation Data Gallery

• 

  WB analysis using 66081-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66081-1-Ig (IFITM2/3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  IHC staining of human liver cancer using 66081-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66081-1-Ig (IFITM2/3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66081-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66081-1-Ig (IFITM2/3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of U2OS using 66081-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using IFITM2/3 antibody (66081-1-Ig, Clone: 2E8D12 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of HeLa using 66081-1-Ig

  1X10^6 HeLa cells were stained with 0.2ug IFITM2/3 antibody (66081-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: U2OS cells, THP-1 cells, HL-60 cells, K-562 cells, Jurkat cells, MCF-7 cells, HeLa cells, LNCaP cells, A549 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: U2OS cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IF: See 6 publications below
• IP: See 1 publications below
• FC: See 2 publications below

Product Information

The immunogen of 66081-1-Ig is IFITM2/3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: IFITM2/3 fusion protein Ag17863
• Full Name: interferon induced transmembrane protein 3 (1-8U)
• Observed molecular weight: 15-20 kDa
• GenBank accession number: BC070243
• Gene symbol: IFITM3
• Gene ID (NCBI): 10410
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IFITM3, also named as interferon-inducible protein 1-8U, belongs to the CD225 family. It is IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus, by inhibiting the early steps of replication. IFITM3 is identified as interferon-induced cellular proteins that restrict infections by retroviruses and filoviruses and of influenza virus and flaviviruses, respectively. IFITM3, the most potent antiviral IFITM, was found to inhibit an uncharacterized early infectious event after VSV endocytosis, but before primary transcription of its viral genome. IFITM proteins are viral restriction factors that can inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. They differentially restrict the entry of a broad range of enveloped viruses, and modulate cellular tropism independently of viral receptor expression. This antibody recognizes both IFITM2 and IFITM3.

Publications

Species	Application	Title
human	FC	Science; IFITM proteins inhibit placental syncytiotrophoblast formation and promote fetal demise.; Authors - Julian Buchrieser
human	IF,FC	EMBO J; Syncytia formation by SARS-CoV-2-infected cells.; Authors - Julian Buchrieser
human	WB,IF	Proc Natl Acad Sci U S A; mTOR inhibitors lower an intrinsic barrier to virus infection mediated by IFITM3.; Authors - Guoli Shi
human	WB	EMBO Rep; Natural mutations in IFITM3 modulate post-translational regulation and toggle antiviral specificity.; Authors - Alex A Compton
human	WB	MBio; Late Endosomal/Lysosomal Cholesterol Accumulation Is a Host Cell-Protective Mechanism Inhibiting Endosomal Escape of Influenza A Virus.; Authors - Alexander Kühnl
human	WB,IF	Front Immunol; Influenza A Virus Facilitates Its Infectivity by Activating p53 to Inhibit the Expression of Interferon-Induced Transmembrane Proteins.; Authors - Bei Wang ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Claudia (Verified Customer) (06-20-2022)

The antibody worked very well for the analysis of IFITM2/3 expression in permanent cell lines.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:200 (immunofluorescence), 1:5000 (Western blot)
• Cell Tissue Type: Permanent cell lines

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