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IFITM2/3 Monoclonal antibody

IFITM2/3 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2E8D12

Cat no : 66081-1-Ig

Synonyms

1 8U, IFITM2/3, IFITM3, IP15



Tested Applications

Positive WB detected inU2OS cells, THP-1 cells, HL-60 cells, K-562 cells, Jurkat cells, MCF-7 cells, HeLa cells, LNCaP cells, A549 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU2OS cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66081-1-Ig targets IFITM2/3 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IFITM2/3 fusion protein Ag17863
Full Name interferon induced transmembrane protein 3 (1-8U)
Observed Molecular Weight 15-20 kDa
GenBank Accession NumberBC070243
Gene Symbol IFITM3
Gene ID (NCBI) 10410
RRIDAB_11182821
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IFITM3, also named as interferon-inducible protein 1-8U, belongs to the CD225 family. It is IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus, by inhibiting the early steps of replication. IFITM3 is identified as interferon-induced cellular proteins that restrict infections by retroviruses and filoviruses and of influenza virus and flaviviruses, respectively. IFITM3, the most potent antiviral IFITM, was found to inhibit an uncharacterized early infectious event after VSV endocytosis, but before primary transcription of its viral genome. IFITM proteins are viral restriction factors that can inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. They differentially restrict the entry of a broad range of enveloped viruses, and modulate cellular tropism independently of viral receptor expression. This antibody recognizes both IFITM2 and IFITM3.

Protocols

Product Specific Protocols
WB protocol for IFITM2/3 antibody 66081-1-IgDownload protocol
IHC protocol for IFITM2/3 antibody 66081-1-IgDownload protocol
IF protocol for IFITM2/3 antibody 66081-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanFC

Science

IFITM proteins inhibit placental syncytiotrophoblast formation and promote fetal demise.

Authors - Julian Buchrieser
humanIF,FC

EMBO J

Syncytia formation by SARS-CoV-2-infected cells.

Authors - Julian Buchrieser
humanWB,IF

Proc Natl Acad Sci U S A

mTOR inhibitors lower an intrinsic barrier to virus infection mediated by IFITM3.

Authors - Guoli Shi
humanWB

EMBO Rep

Natural mutations in IFITM3 modulate post-translational regulation and toggle antiviral specificity.

Authors - Alex A Compton
humanWB

MBio

Late Endosomal/Lysosomal Cholesterol Accumulation Is a Host Cell-Protective Mechanism Inhibiting Endosomal Escape of Influenza A Virus.

Authors - Alexander Kühnl
humanWB,IF

Front Immunol

Influenza A Virus Facilitates Its Infectivity by Activating p53 to Inhibit the Expression of Interferon-Induced Transmembrane Proteins.

Authors - Bei Wang
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Claudia (Verified Customer) (06-20-2022)

The antibody worked very well for the analysis of IFITM2/3 expression in permanent cell lines.

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:200 (immunofluorescence), 1:5000 (Western blot)
  • Cell Tissue Type: Permanent cell lines