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IFITM2/3 Monoclonal antibody
IFITM2/3 Monoclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
WB, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
CloneNo.
2E8D12
Cat no : 66081-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | U2OS cells, THP-1 cells, HL-60 cells, K-562 cells, Jurkat cells, MCF-7 cells, HeLa cells, LNCaP cells, A549 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | U2OS cells |
| Positive FC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 8 publications below |
| IF | See 6 publications below |
| IP | See 1 publications below |
| FC | See 2 publications below |
Product Information
The immunogen of 66081-1-Ig is IFITM2/3 Fusion Protein expressed in E. coli.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | IFITM2/3 fusion protein Ag17863 |
| Full Name | interferon induced transmembrane protein 3 (1-8U) |
| Observed molecular weight | 15-20 kDa |
| GenBank accession number | BC070243 |
| Gene symbol | IFITM3 |
| Gene ID (NCBI) | 10410 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IFITM3, also named as interferon-inducible protein 1-8U, belongs to the CD225 family. It is IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus, by inhibiting the early steps of replication. IFITM3 is identified as interferon-induced cellular proteins that restrict infections by retroviruses and filoviruses and of influenza virus and flaviviruses, respectively. IFITM3, the most potent antiviral IFITM, was found to inhibit an uncharacterized early infectious event after VSV endocytosis, but before primary transcription of its viral genome. IFITM proteins are viral restriction factors that can inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. They differentially restrict the entry of a broad range of enveloped viruses, and modulate cellular tropism independently of viral receptor expression. This antibody recognizes both IFITM2 and IFITM3.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IFITM2/3 antibody 66081-1-Ig | Download protocol |
| IHC protocol for IFITM2/3 antibody 66081-1-Ig | Download protocol |
| IF protocol for IFITM2/3 antibody 66081-1-Ig | Download protocol |
| FC protocol for IFITM2/3 antibody 66081-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
mBio IFITM3 Reduces Retroviral Envelope Abundance and Function and Is Counteracted by glycoGag. | ||
Front Microbiol Inhibition of Arenavirus Entry and Replication by the Cell-Intrinsic Restriction Factor ZMPSTE24 Is Enhanced by IFITM Antiviral Activity. | ||
J Virol Escape of HIV-1 envelope glycoprotein from the restriction of infection by IFITM3. | ||
J Virol Human Cytomegalovirus Exploits Interferon-Induced Transmembrane Proteins to Facilitate Morphogenesis of the Virion Assembly Compartment.
| ||
Front Immunol Interferon-Induced Transmembrane Protein 3 Inhibits Hantaan Virus Infection, and Its Single Nucleotide Polymorphism rs12252 Influences the Severity of Hemorrhagic Fever with Renal Syndrome. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Claudia (Verified Customer) (06-20-2022) | The antibody worked very well for the analysis of IFITM2/3 expression in permanent cell lines.
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