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IFITM2/3 Antibody

IFITM2/3 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2E8D12

Cat no : 66081-1-Ig

Synonyms

1 8U, IFITM3, IP15



Tested Applications

Positive WB detected inU2OS cells, THP-1 cells, HL-60 cells, K-562 cells, Jurkat cells, MCF-7 cells, HeLa cells, LNCaP cells, A549 cells, HeLa cells, human placenta tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The 66081-1-Ig immunogen is Fusion Protein IFITM2/3 protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IFITM2/3 fusion protein Ag17863
Full Name interferon induced transmembrane protein 3 (1-8U)
Calculated molecular weight
Observed molecular weight 14 kDa
GenBank accession number BC070243
Gene symbol IFITM3
Gene ID (NCBI) 10410
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IFITM3, also named as interferon-inducible protein 1-8U, belongs to the CD225 family. It is IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus, by inhibiting the early steps of replication. IFITM3 is identified as interferon-induced cellular proteins that restrict infections by retroviruses and filoviruses and of influenza virus and flaviviruses, respectively. IFITM3, the most potent antiviral IFITM, was found to inhibit an uncharacterized early infectious event after VSV endocytosis, but before primary transcription of its viral genome. IFITM proteins are viral restriction factors that can inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. They differentially restrict the entry of a broad range of enveloped viruses, and modulate cellular tropism independently of viral receptor expression. This antibody recognizes both IFITM2 and IFITM3.

Protocols

Product Specific Protocols
WB protocol for IFITM2/3 antibody 66081-1-IgDownload protocol
IHC protocol for IFITM2/3 antibody 66081-1-IgDownload protocol
IF protocol for IFITM2/3 antibody 66081-1-IgDownload protocol
FC protocol for IFITM2/3 antibody 66081-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

mBio

IFITM3 Reduces Retroviral Envelope Abundance and Function and Is Counteracted by glycoGag.

Authors - Yadvinder S Ahi
humanWB

Int J Mol Sci

Analysis of IFITM-IFITM Interactions by a Flow Cytometry-Based FRET Assay.

Authors - Michael Winkler
humanWB, IF

J Virol

Human Cytomegalovirus Exploits Interferon-Induced Transmembrane Proteins to Facilitate Morphogenesis of the Virion Assembly Compartment.

Authors - Maorong Xie
  • KD Validated
HUMANWB,IF

Front Immunol

Interferon-Induced Transmembrane Protein 3 Inhibits Hantaan Virus Infection, and Its Single Nucleotide Polymorphism rs12252 Influences the Severity of Hemorrhagic Fever with Renal Syndrome.

Authors - Zheng Xu-Yang
humanWB,IF,FC

Front Immunol

Influenza A Virus Facilitates Its Infectivity by Activating p53 to Inhibit the Expression of Interferon-Induced Transmembrane Proteins.

Authors - Bei Wang
  • KD Validated
humanWB

MBio

Late Endosomal/Lysosomal Cholesterol Accumulation Is a Host Cell-Protective Mechanism Inhibiting Endosomal Escape of Influenza A Virus.

Authors - Alexander Kühnl