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  • KD/KO Validated

IFITM2/3 Monoclonal antibody

IFITM2/3 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1









Cat no : 66081-1-Ig


1 8U, IFITM2/3, IFITM3, IP15

Tested Applications

Positive WB detected inU2OS cells, THP-1 cells, HL-60 cells, K-562 cells, Jurkat cells, MCF-7 cells, HeLa cells, LNCaP cells, A549 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU2OS cells
Positive FC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite®488, CoraLite®555 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66081-1-Ig is IFITM2/3 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IFITM2/3 fusion protein Ag17863
Full Name interferon induced transmembrane protein 3 (1-8U)
Observed molecular weight 15-20 kDa
GenBank accession numberBC070243
Gene symbol IFITM3
Gene ID (NCBI) 10410
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IFITM3, also named as interferon-inducible protein 1-8U, belongs to the CD225 family. It is IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus, by inhibiting the early steps of replication. IFITM3 is identified as interferon-induced cellular proteins that restrict infections by retroviruses and filoviruses and of influenza virus and flaviviruses, respectively. IFITM3, the most potent antiviral IFITM, was found to inhibit an uncharacterized early infectious event after VSV endocytosis, but before primary transcription of its viral genome. IFITM proteins are viral restriction factors that can inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. They differentially restrict the entry of a broad range of enveloped viruses, and modulate cellular tropism independently of viral receptor expression. This antibody recognizes both IFITM2 and IFITM3.


Product Specific Protocols
WB protocol for IFITM2/3 antibody 66081-1-IgDownload protocol
IHC protocol for IFITM2/3 antibody 66081-1-IgDownload protocol
IF protocol for IFITM2/3 antibody 66081-1-IgDownload protocol
FC protocol for IFITM2/3 antibody 66081-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols




IFITM3 Reduces Retroviral Envelope Abundance and Function and Is Counteracted by glycoGag.

Authors - Yadvinder S Ahi

Int J Mol Sci

Analysis of IFITM-IFITM Interactions by a Flow Cytometry-Based FRET Assay.

Authors - Michael Winkler

Front Microbiol

Inhibition of Arenavirus Entry and Replication by the Cell-Intrinsic Restriction Factor ZMPSTE24 Is Enhanced by IFITM Antiviral Activity.

Authors - Robert J Stott-Marshall

J Virol

Escape of HIV-1 envelope glycoprotein from the restriction of infection by IFITM3.

Authors - Aurélie Drouin
humanWB, IF

J Virol

Human Cytomegalovirus Exploits Interferon-Induced Transmembrane Proteins to Facilitate Morphogenesis of the Virion Assembly Compartment.

Authors - Maorong Xie
  • KD Validated

Front Immunol

Interferon-Induced Transmembrane Protein 3 Inhibits Hantaan Virus Infection, and Its Single Nucleotide Polymorphism rs12252 Influences the Severity of Hemorrhagic Fever with Renal Syndrome.

Authors - Zheng Xu-Yang


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Claudia (Verified Customer) (06-20-2022)

The antibody worked very well for the analysis of IFITM2/3 expression in permanent cell lines.

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:200 (immunofluorescence), 1:5000 (Western blot)
  • Cell Tissue Type: Permanent cell lines