IGFALS Polyclonal antibody

• 

  WB analysis of human plasma using 24545-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24545-1-AP (IGFALS antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

IGFALS Polyclonal Antibody for WB, ELISA

Cat No. 24545-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

ALS, Insulin-like growth factor-binding protein complex acid labile subunit

---

Tested Applications

• Positive WB detected in: human plasma

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24545-1-AP targets IGFALS in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag21696

  Product name: Recombinant human IGFALS protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 43-378 aa of BC025681

  Sequence: AACVCSYDDDADELSVFCSSRNLTRLPDGVPGGTQALWLDGNNLSSVPPAAFQNLSSLGFLNLQGGQLGSLEPQALLGLENLCHLHLERNQLRSLALGTFAHTPALASLGLSNNRLSRLEDGLFEGLGSLWDLNLGWNSLAVLPDAAFRGLGSLRELVLAGNRLAYLQPALFSGLAELRELDLSRNALRAIKANVFVQLPRLQKLYLDRNLIAAVAPGAFLGLKALRWLDLSHNRVAGLLEDTFPGLLGLRVLRLSHNAIASLRPRTFKDLHFLEELQLGHNRIRQLAERSFEGLGQLEVLTLDHNQLQEVKAGAFLGLTNMAVMNLSGNCLRNLP

• Full Name: insulin-like growth factor binding protein, acid labile subunit
• Calculated Molecular Weight: 605 aa, 66 kDa
• Observed Molecular Weight: 85 kDa
• GenBank Accession Number: BC025681
• Gene Symbol: IGFALS
• Gene ID (NCBI): 3483
• RRID: AB_3085738
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P35858
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

THE ACID-LABILE SUBUNIT (ALS), a liver-derived, GH-dependent, 85-kD glycoprotein, is a key component of the 150-kD IGF circulating ternary complex(PMID: 17726072). The protein encoded by this gene is a serum protein that binds insulin-like growth factors, increasing their half-life and their vascular localization. Production of the encoded protein, which contains twenty leucine-rich repeats, is stimulated by growth hormone. Defects in this gene are a cause of acid-labile subunit deficiency, which maifests itself in a delayed and slow puberty.