Product Information
This product is a semi-solid hydrophobic immunohistochemical pen. It is suitable for marking around tissue sections on glass slides to create a hydrophobic isolation area that prevents liquid from spreading or running off the edges. This product does not contain volatile hazardous substances such as xylene and resists drying during storage. It is ready to use immediately after opening, requiring no waiting or priming. Each pen can produce approximately 500 circles of 1cm diameter.
Note: This product is not recommended for immunofluorescence due to auto-fluorescence from the materials in the pen core.
Storage
Store at 4-30℃. Note that this product is not heat resistant and will melt at temperatures above 45°C.
Shelf life: 3 years from the date of receipt.
Usage
1. After deparaffinization/tissue preparation and before applying the blocking reagent or primary antibody, dry any remaining fluid around the tissue with absorbent paper.
2. After removing the pen cap, twist the pen to extend the pen core about 5 mm. Extending the core of the pen too far can lead to bending or breakage during use.
3. Use the IHC pen to draw a closed hydrophobic circle around the tissue before applying IHC reagents. If the tip of the pen is too blunt and inconvenient to use, you can use a utility knife to lightly sharpen it again. Replace the pen cap for storage.
Cautions
1. This product is not resistant to high temperature and it may melt above 45°C, leading to deformation of the core material and melting or spreading of the circle applied to a slide.
2. This product can be dissolved in ethanol, methanol, xylene and acetone. The tissue should be deparaffinized and rehydrated before the IHC pen is applied to the slide (otherwise the IHC pen marking will disappear).
Cited in Article as
PR30013, Hydrophobic IHC pen, Proteintech, IL, USA
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Pratiksha (Verified Customer) (03-28-2026) | The hydrophobic IHC pen works to mark the borders and holds the solutions from running off the edges for the first few washes but slowly starts peeling off with continued washes and incubations especially if triton x is used in multiple steps of the protocol.
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FH Georgia (Verified Customer) (01-20-2026) | Hydrophobic pen worked well on slides for marking around mouse brain tissue sections
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