IKBKE Monoclonal antibody

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  WB analysis using 68531-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68531-1-Ig (IKBKE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68531-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68531-1-Ig (IKBKE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

IKBKE Monoclonal Antibody for WB, ELISA

Cat No. 68531-1-Ig

Clone No.3E4H7

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Synonyms

I kappa B kinase epsilon, IKBKE, IKK E, IKK epsilon, IKK i, IKKE, IKKI, Inducible I kappa B kinase, KIAA0151

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Tested Applications

• Positive WB detected in: SK-BR-3 cells, Jurkat cells, MCF-7 cells, HeLa cells, Raji cells, Ramos cells, Daudi cells, HL-60 cells, THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68531-1-Ig targets IKBKE in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag30374

  Product name: Recombinant human IKBKE protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 259-394 aa of BC107812

  Sequence: SVAPRHQEYLFEGHLCVLEPSVSAQHIAHTTASSPLTLFSTAIPKGLAFRDPALDVPKFVPKVDLQADYNTAKGVLGAGYQALRLARALLDGQELMFRGLHWVMEVLQATCRRTLEVARTSLLYLSSSLGTERFSS

• Full Name: inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon
• Calculated Molecular Weight: 716 aa, 80 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: BC107812
• Gene Symbol: IKBKE
• Gene ID (NCBI): 9641
• RRID: AB_3085239
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q14164
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IKBKE is a serine/threonine kinase that plays an essential role in regulating inflammatory responses to viral infection, through the activation of the type I IFN, NF-kappa-B and STAT signaling. It is also involved in TNFA and inflammatory cytokines, like Interleukin-1, signaling.