FITC Plus Anti-Mouse IL-12/IL-23 p40 (C17.8)

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  FC experiment of mouse peritoneal macrophages using FITC-65097

  1X10^6 IFN-γ primed Balb/c mouse peritoneal macrophages were unstimulated or stimulated with LPS in the presence of a protein transport inhibitor and then intracellularly stained with 0.5 ug FITC Plus Anti-Mouse IL-12/IL-23 p40 (FITC-65097, Clone:C17.8). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

IL-12/IL-23 p40 Monoclonal Antibody for FC (Intra)

Cat No. FITC-65097

Clone No.C17.8

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse

Applications

FC (Intra)

Synonyms

Il 12b, Il 12p40, Il12b, Il12p40, interleukin 12b, p40

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Tested Applications

• Positive FC (Intra) detected in: mouse peritoneal macrophages
• Positive FC detected in: mouse peritoneal macrophages

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.5 ug per 10^6 cells in 100 μl suspension
• Flow Cytometry (FC): FC :

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

FITC-65097 targets IL-12/IL-23 p40 in FC (Intra) applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Rat / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: CHO expressed, recombinant mouse IL-12 p70
• Full Name: interleukin 12b
• GenBank Accession Number: BC103608
• Gene Symbol: Il12b
• Gene ID (NCBI): 16160
• RRID: AB_3084997
• Conjugate: FITC Plus Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 495 nm / 524 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Interleukin (IL)-12 and IL-23 are heterodimeric cytokines that share a common p40 subunit (PMID: 11114383). IL-12 is composed of the IL-12 p40 subunit linked to the IL-12 p35 subunit, and the heterodimer signals through the IL-12 receptor (IL-12R), which comprises the IL-12Rβ1 and IL-12Rβ2 subunits. IL-23 is composed of the IL-23 p19 subunit and the IL-12 p40 (IL-12/23p40) subunit, which signals through IL-23R and IL-12Rβ1 (PMID: 11114383; 26121196 ). IL-12/IL-23 p40 also exists as a monomer and as a homodimer which can act as a potent IL-12 antagonist (PMID: 8958912; 18783467). IL-12/IL-23 p40 is produced by antigen-presenting cells, such as dendritic cells (DCs), monocytes, macrophages, neutrophils and, to a lesser extent, B cells (PMID: 20476918).