CoraLite® Plus 647 Anti-Mouse IL-12/IL-23 p40 (C17.8)

• 

  FC experiment of mouse peritoneal macrophages using CL647-65097

  1X10^6 IFN-γ primed murine peritoneal macrophages stimulated with LPS were surface stained with PE Anti-Mouse CD11b (M1/70), then fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer, and intracellularly stained with 0.5 ug CoraLite® Plus 647 Anti-Mouse IL-12/IL-23 p40 (CL647-65097, Clone: C17.8) or 0.5 ug CoraLite® Plus 647 Rat IgG2a Isotype Control (2A3) (CL647-65209, Clone: 2A3).

IL-12/IL-23 p40 Monoclonal Antibody for FC (Intra)

Cat No. CL647-65097

Clone No.C17.8

Host / Isotype

Rat / IgG2a, kappa

Reactivity

mouse

Applications

FC (Intra)

Synonyms

Il12b, C17.8, Cytotoxic lymphocyte maturation factor 40 kDa subunit, Il 12b, Il 12p40

---

Tested Applications

• Positive FC (Intra) detected in: IFN-γ mouse peritoneal macrophages

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.5 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65097 targets IL-12/IL-23 p40 in FC (Intra) applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rat / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CHO expressed, recombinant mouse IL-12 p70

• Full Name: interleukin 12b
• GenBank Accession Number: BC103608
• Gene Symbol: Il12b
• Gene ID (NCBI): 16160
• RRID: AB_2920255
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P43432
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.