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Anti-Mouse IL-12/IL-23 p40 (C17.8)

IL-12/IL-23 p40 Monoclonal Antibody for FC (Intra)

Host / Isotype

Rat / IgG2a, kappa

Reactivity

mouse

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

C17.8

Cat no : 65097-1-Ig

Synonyms

Il12b, Il-12b, IL-12 p40, Il 12p40, Il 12b

Validation Data Gallery

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  • Flow cytometry (FC) experiment of RAW 264.7 cells using Anti-Mouse IL-12/IL-23 p40 (C17.8) (65097-1-Ig)

    FC experiment of RAW 264.7 using 65097-1-Ig

    1X10^6 untreated or IFNγ, LPS, BFA and Monensin treated RAW 264.7 cells were intracellularly stained with 0.5 ug Anti-Mouse IL-12/IL-23 p40 (65097-1-Ig, Clone: C17.8) and FITC anti-rat IgG2a Antibody at dilution 1:100. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC (Intra) detected inIFNγ, LPS, BFA and Monensin treated RAW 264.7 cells

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65097-1-Ig targets IL-12/IL-23 p40 in FC (Intra) applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rat / IgG2a, kappa
Class Monoclonal
Type Antibody
Immunogen CHO expressed, recombinant mouse IL-12 p70
Full Name interleukin 12b
GenBank Accession NumberBC103608
Gene Symbol Il12b
Gene ID (NCBI) 16160
RRIDAB_2918399
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

Interleukin (IL)-12 and IL-23 are heterodimeric cytokines that share a common p40 subunit (PMID: 11114383). IL-12 is composed of the IL-12 p40 subunit linked to the IL-12 p35 subunit, and the heterodimer signals through the IL-12 receptor (IL-12R), which comprises the IL-12Rβ1 and IL-12Rβ2 subunits. IL-23 is composed of the IL-23 p19 subunit and the IL-12 p40 (IL-12/23p40) subunit, which signals through IL-23R and IL-12Rβ1 (PMID: 11114383; 26121196 ). IL-12/IL-23 p40 also exists as a monomer and as a homodimer which can act as a potent IL-12 antagonist (PMID: 8958912; 18783467). IL-12/IL-23 p40 is produced by antigen-presenting cells, such as dendritic cells (DCs), monocytes, macrophages, neutrophils and, to a lesser extent, B cells (PMID: 20476918).

Protocols

Product Specific Protocols
FC protocol for IL-12/IL-23 p40 antibody 65097-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols