Anti-Rat IL-12A/IL-12 p35 Rabbit Recombinant Antibody

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  FC experiment of Transfected HEK-293T using 98594-2-RR

  1x10^6 HEK-293T cells (left) or IL-12A/IL-12 p35 Transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Rat IL-12A/IL-12 p35 Rabbit RecAb (98594-2-RR, Clone:250739E10) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

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  FC experiment of C6 using 98594-2-RR

  1x10^6 C6 cells were intracellularly stained with 0.25 ug Anti-Rat IL-12A/IL-12 p35 Rabbit RecAb (98594-2-RR, Clone: 250739E10) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

• 

  FC experiment of Transfected HEK-293T using 98594-2-RR

  1x10^6 HEK-293T cells (left) or IL-12A/IL-12 p35 Transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Rat IL-12A/IL-12 p35 Rabbit RecAb (98594-2-RR, Clone: 250739E10) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

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  Affinity of 98594-2-RR

  Biolayer interferometry (BLl) kinetic assays of 98594-2-RR against Rat IL-12A/IL-12 p35 were performed. The affinity constant is below 1 pM.

IL-12A/IL-12 p35 Uni-rAb^® Recombinant Antibody for FC (Intra)

Cat No. 98594-2-RR

Clone No.250739E10

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

FC (Intra)

Synonyms

CLMF p35, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL-12 subunit p35, Il12a, IL-12A

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Tested Applications

• Positive FC (Intra) detected in: C6 cells, Transfected HEK-293T cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98594-2-RR targets IL-12A/IL-12 p35 in FC (Intra) applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg3823

  Product name: Recombinant Rat IL-12A protein (rFc Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 23-215 aa of NM_053390.1

  Sequence: RVIPVSGPAKCLNQSQNLLKTTDDMVRTAREKLKHYSCTAGDIDHEDITRDKTSTLEACLPLELHKNESCLATKETSSIIRGSCLPPQKTSLMMTLCLGSIYEDLKMYQSEFQAINAALQSHNHQQITLDRNMLMAIDELMRSLNHSGETLHQKAPMGEADPYRVKMKLCILLHAFSTRVMTINRVMNYLSSS

• Full Name: interleukin 12a
• Calculated Molecular Weight: 24 kDa
• GenBank Accession Number: NM_053390.1
• Gene Symbol: Il12a
• Gene ID (NCBI): 84405
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9R103
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

IL-12 is a heterodimeric cytokine composed of two glycosylated and disulfide-linked subunits (p40 cysteine-linked to p35). IL-12 is a potent regulator of cell mediated immune responses and it induces IFN-gamma production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes, and connective tissue type mast cells (PMID: 24821971; 7612223).