IL-27A p28 Recombinant monoclonal antibody

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• IL-27A p28 Recombinant monoclonal antibody (87540-1-RR)

• 

  WB analysis using 87540-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87540-1-RR (IL-27 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

IL-27A p28 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87540-1-RR

Clone No.252839D4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

IL27, IL-27, IL 27, IL 27 A, IL 27 subunit alpha

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Tested Applications

• Positive WB detected in: HeLa cells, Daudi cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87540-1-RR targets IL-27A p28 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo:

  Product name: HumanKine® recombinant human IL-27 protein

  Source: -derived,

  Tag:

  Domain: of

  Sequence:

• Full Name: interleukin 27
• Observed Molecular Weight: 27 kDa
• Gene Symbol: IL-27
• Gene ID (NCBI): 246778
• RRID: AB_3745610
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8NEV9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IL-27 is a heterodimeric cytokine composed of two subunits: IL-27a and IL-27b. IL-27 acts on various cell types, including T cells, B cells, macrophages, dendritic cells, natural killer (NK) cells and non-hematopoietic cells. IL-27 plays a critical role in the early regulation of T helper type 1 initiation, and enhances proliferation of naive CD4+T cells and naive B cells. It, however, also exerts anti-inflammatory functions by inhibiting the development of Th17 cells and inducing IL-10 producing type 1 regulatory T cells. IL-27 is a potentially promising cytokine for therapeutic approaches on various human diseases.