IL-23R Polyclonal antibody

IL-23R Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 27163-1-AP

Synonyms

IL 23 receptor, IL 23R, IL23R, IL-23R, interleukin 23 receptor

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Validation Data Gallery

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  WB analysis using 27163-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27163-1-AP (IL23R antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 27163-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27163-1-AP (IL23R antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 27163-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27163-1-AP (IL23R antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 27163-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27163-1-AP (IL23R antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Jurkat cells, A431 cells, RAW 264.7 cells, Raji cells, THP-1 cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

The immunogen of 27163-1-AP is IL-23R Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IL-23R fusion protein Ag25939
• Full Name: interleukin 23 receptor
• Calculated Molecular Weight: 72 kDa
• Observed Molecular Weight: 65-75 kDa
• GenBank Accession Number: NM_144701
• Gene Symbol: IL23R
• Gene ID (NCBI): 149233
• RRID: AB_2880783
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Front Immunol; IL-17 Aggravates Pseudomonas aeruginosa Airway Infection in Acute Exacerbations of Chronic Obstructive Pulmonary Disease.; Authors - Fengming Ding
rat	WB	Naunyn Schmiedebergs Arch Pharmacol; Short-term pretreatment of naringin isolated from Citrus wilsonii Tanaka attenuates rat myocardial ischemia/reperfusion injury.; Authors - Wenting Liu