CXCR2 Polyclonal antibody

CXCR2 Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IF,ELISA

Conjugate

Unconjugated

Cat no : 20634-1-AP

Synonyms

CD182, CDw128b, CMKAR2, CXC R2, CXCR 2, CXCR2, GRO/MGSA receptor, IL 8 receptor type 2, IL 8R B, IL8R2, IL8RA, IL8RB, interleukin 8 receptor, beta



Tested Applications

Positive WB detected inL02 cells, COLO 320 cells
Positive IP detected inHepG2 cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20634-1-AP is CXCR2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CXCR2 fusion protein Ag14670
Full Name interleukin 8 receptor, beta
Calculated molecular weight 360 aa, 41 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC037961
Gene symbol IL8RB
Gene ID (NCBI) 3579
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CXCR2 antibody 20634-1-APDownload protocol
IHC protocol for CXCR2 antibody 20634-1-APDownload protocol
IF protocol for CXCR2 antibody 20634-1-APDownload protocol
IP protocol for CXCR2 antibody 20634-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

JCI Insight

Neutralization of IL-8 decreases tumor PMN-MDSCs and reduces mesenchymalization of claudin-low triple-negative breast cancer.

Authors - Charli Dominguez
mouseWB

Cytokine

IL-8 exacerbates alcohol-induced fatty liver disease via the Akt/HIF-1α pathway in human IL-8-expressing mice.

Authors - Zhihao Wang
mouseWB

Cell Commun Signal

XIAOPI formula inhibits the pre-metastatic niche formation in breast cancer via suppressing TAMs/CXCL1 signaling.

Authors - Yifeng Zheng