• Featured Product
  • KD/KO Validated

CXCR2 Polyclonal antibody

CXCR2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 20634-1-AP

Synonyms

CD182, CDw128b, CMKAR2, CXC R2, CXCR 2, CXCR2, GRO/MGSA receptor, IL 8 receptor type 2, IL 8R B, IL8R2, IL8RA, IL8RB, IL-8RB, interleukin 8 receptor, beta



Tested Applications

Positive WB detected inL02 cells, COLO 320 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20634-1-AP targets CXCR2 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CXCR2 fusion protein Ag14670
Full Name interleukin 8 receptor, beta
Calculated Molecular Weight 360 aa, 41 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC037961
Gene Symbol IL8RB
Gene ID (NCBI) 3579
RRIDAB_10693624
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IL8RB, also named as CXCR2, GRO/MGSA receptor, IL-8 receptor type 2, CDw128b, and CD182, belongs to the G-protein coupled receptor 1 family. It is a receptor for interleukin-8 which is a powerful neutrophil chemotactic factor. The binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. It binds to IL-8 with high affinity. IL8RB also binds with high affinity to CXCL3, GRO/MGSA, and NAP-2.

Protocols

Product Specific Protocols
WB protocol for CXCR2 antibody 20634-1-APDownload protocol
IHC protocol for CXCR2 antibody 20634-1-APDownload protocol
IF protocol for CXCR2 antibody 20634-1-APDownload protocol
IP protocol for CXCR2 antibody 20634-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cell Rep

Prostate cancer cell-derived exosomal IL-8 fosters immune evasion by disturbing glucolipid metabolism of CD8+ T cell

Authors - Fan Xu
humanWB

Sci Signal

Skewing cPLA2α activity toward oxoeicosanoid production promotes neutrophil N2 polarization, wound healing, and the response to sepsis

Authors - Kenneth D Maus
humanWB

Int J Mol Sci

MyD88 in Macrophages Enhances Liver Fibrosis by Activation of NLRP3 Inflammasome in HSCs.

Authors - Shuang Ge
humanIF

Front Immunol

CXCL5 Downregulation in Villous Tissue Is Correlated With Recurrent Spontaneous Abortion

Authors - Sainan Zhang
humanWB,IHC,IF

Pharmaceuticals (Basel)

CXCL8 Promotes Endothelial-to-Mesenchymal Transition of Endothelial Cells and Protects Cells from Erastin-Induced Ferroptosis via CXCR2-Mediated Activation of the NF-κB Signaling Pathway

Authors - Hai-Zhou Ji
  • KD Validated
humanIF

JCI Insight

Neutralization of IL-8 decreases tumor PMN-MDSCs and reduces mesenchymalization of claudin-low triple-negative breast cancer.

Authors - Charli Dominguez