• Featured Product
  • KD/KO Validated

IMPDH2 Polyclonal antibody

IMPDH2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 12948-1-AP

Synonyms

IMP dehydrogenase 2, IMPD 2, IMPD2, IMPDH 2, IMPDH II, IMPDH2



Tested Applications

Positive WB detected inA549 cells, rat heart tissue, HeLa cells, HepG2 cells, K-562 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12948-1-AP targets IMPDH2 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IMPDH2 fusion protein Ag3632
Full Name IMP (inosine monophosphate) dehydrogenase 2
Calculated molecular weight 514 aa, 56 kDa
Observed molecular weight 56 kDa
GenBank accession numberBC012840
Gene symbol IMPDH2
Gene ID (NCBI) 3615
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IMPDH2, also named as IMPD2, belongs to the IMPDH/GMPR family. It is a rate limiting enzyme in the de novo synthesis of guanine nucleotides and therefore is involved in the regulation of cell growth. It may also have a role in the development of malignancy and the growth progression of some tumors. IMPDH2 is a key enzyme in the purine nucleotide biosynthetic pathway and constitutes a pivotal biological target for immunosuppressant and antiviral drugs. (PMID:21181270) IMPDH2 is highly enriched in RR(filamentous rods and rings). This antibody may has cross reaction with IMPDH1 due to the high homology.

Protocols

Product Specific Protocols
WB protocol for IMPDH2 antibody 12948-1-APDownload protocol
IHC protocol for IMPDH2 antibody 12948-1-APDownload protocol
IF protocol for IMPDH2 antibody 12948-1-APDownload protocol
IP protocol for IMPDH2 antibody 12948-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Front Immunol

Immune Response-Dependent Assembly of IMP Dehydrogenase Filaments.

Authors - S John Calise
mouseIF

Antiviral Res

PMID: 30605724

Authors - Gerson Dierley Keppeke
ELISA

Immunol Res

PMID: 24845459

Authors - Gerson Dierley Keppeke
humanWB

Cell Death Dis

Rewiring of purine metabolism in response to acidosis stress in glioma stem cells.

Authors - Xiaoyu Xu
humanWB,IF

FEBS J

Inter-filament interaction between IMPDH and CTPS cytoophidia.

Authors - Chia-Chun Chang
humanIHC

Int J Colorectal Dis

Identification of IMPDH2 as a tumor-associated antigen in colorectal cancer using immunoproteomics analysis.

Authors - He Yujun Y