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IMPDH2 Polyclonal antibody
IMPDH2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, RIP, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 12948-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A549 cells, rat heart tissue, HeLa cells, HepG2 cells, K-562 cells |
Positive IP detected in | K-562 cells |
Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 4 publications below |
WB | See 22 publications below |
IHC | See 3 publications below |
IF | See 27 publications below |
IP | See 1 publications below |
ELISA | See 1 publications below |
RIP | See 1 publications below |
Product Information
12948-1-AP targets IMPDH2 in WB, RIP, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, zebrafish |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | IMPDH2 fusion protein Ag3632 |
Full Name | IMP (inosine monophosphate) dehydrogenase 2 |
Calculated molecular weight | 514 aa, 56 kDa |
Observed molecular weight | 56 kDa |
GenBank accession number | BC012840 |
Gene symbol | IMPDH2 |
Gene ID (NCBI) | 3615 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IMPDH2, also named as IMPD2, belongs to the IMPDH/GMPR family. It is a rate limiting enzyme in the de novo synthesis of guanine nucleotides and therefore is involved in the regulation of cell growth. It may also have a role in the development of malignancy and the growth progression of some tumors. IMPDH2 is a key enzyme in the purine nucleotide biosynthetic pathway and constitutes a pivotal biological target for immunosuppressant and antiviral drugs. (PMID:21181270) IMPDH2 is highly enriched in RR(filamentous rods and rings). This antibody may has cross reaction with IMPDH1 due to the high homology.
Protocols
Product Specific Protocols | |
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WB protocol for IMPDH2 antibody 12948-1-AP | Download protocol |
IHC protocol for IMPDH2 antibody 12948-1-AP | Download protocol |
IF protocol for IMPDH2 antibody 12948-1-AP | Download protocol |
IP protocol for IMPDH2 antibody 12948-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Mol Cell ERK2 Phosphorylates PFAS to Mediate Posttranslational Control of De Novo Purine Synthesis. | ||
Mol Ther IMPDH1/YB-1 Positive Feedback Loop Assembles Cytoophidia and Represents a Therapeutic Target in Metastatic Tumors.
| ||
Cancer Lett Circular RNA circPFKP promotes cell proliferation by activating IMPDH2 in prostate cancer. | ||
Elife SARS-CoV-2 Nsp14 mediates the effects of viral infection on the host cell transcriptome. |