ING5 Polyclonal antibody, PBS Only

• 

  WB analysis of Jurkat using 10665-1-AP (same clone as 10665-1-PBS)

  WB result of ING5 antibody (10665-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ING5 transfected Jurkat cells. This data was developed using the same antibody clone with 10665-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 10665-1-AP (same clone as 10665-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10665-1-AP (ING5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10665-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 10665-1-AP (same clone as 10665-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10665-1-AP (ING5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10665-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 10665-1-AP (same clone as 10665-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10665-1-AP (ING5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10665-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse kidney using 10665-1-AP (same clone as 10665-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10665-1-AP (ING5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10665-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 10665-1-AP (same clone as 10665-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10665-1-AP (ING5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10665-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HEK-293 using 10665-1-AP (same clone as 10665-1-PBS)

  IP result of anti-ING5 (IP:10665-1-AP, 4ug; Detection:10665-1-AP 1:700) with HEK-293 cells lysate 2560ug. This data was developed using the same antibody clone with 10665-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 10665-1-AP (same clone as 10665-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10665-1-AP (ING5 antibody) at dilution of 1:100 (under 25x lens). This data was developed using the same antibody clone with 10665-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid cancer using 10665-1-AP (same clone as 10665-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10665-1-AP (ING5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10665-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 10665-1-AP (same clone as 10665-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10665-1-AP (ING5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10665-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat skin using 10665-1-AP (same clone as 10665-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 10665-1-AP (ING5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10665-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 10665-1-AP (same clone as 10665-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10665-1-AP (ING5 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10665-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 10665-1-AP (same clone as 10665-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10665-1-AP (ING5 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10665-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

ING5 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 10665-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Inhibitor of growth protein 5, p28ING5

---

Product Information

10665-1-PBS targets ING5 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1059

  Product name: Recombinant human ING5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-226 aa of BC005370

  Sequence: MATAMYLEHYLDSIENLPCELQRNFQLMRELDQRTEDKKAEIDILAAEYISTVKTLSPDQRVERLQKIQNAYSKCKEYSDDKVQLAMQTYEMVDKHIRRLDADLARFEADLKDKMEGSDFESSGGRGLKKGRGQKEKRGSRGRGRRTSEEDTPKKKKHKGGSEFTDTILSVHPSDVLDMPVDPNEPTYCLCHQVSYGEMIGCDNPDCPIEWFHFACVDLTTKPKGK

• Full Name: inhibitor of growth family, member 5
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 28-32 kDa
• GenBank Accession Number: BC005370
• Gene Symbol: ING5
• Gene ID (NCBI): 84289
• RRID: AB_2280522
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8WYH8
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ING5, also named as p28ING5, belongs to the ING family. It is a component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. ING5 is a component of the MOZ/MORF complex which has a histone H3 acetyltransferase activity. Through chromatin acetylation it may regulate DNA replication and may function as a transcriptional coactivator. (PMID:12750254) Altered ING5 expression and its shift into the cytoplasm may have an impact on the malignant transformation of colorectal epithelial cells and should be considered as a biomarker for colorectal carcinogenesis. (PMID:21193223)