Tested Applications
| Positive WB detected in | Jurkat cells, PC-3 cells, mouse kidney tissue, HEK-293 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human colon cancer tissue, human ovary tumor tissue, human thyroid cancer tissue, mouse kidney tissue, rat skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10665-1-AP targets ING5 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1059 Product name: Recombinant human ING5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-226 aa of BC005370 Sequence: MATAMYLEHYLDSIENLPCELQRNFQLMRELDQRTEDKKAEIDILAAEYISTVKTLSPDQRVERLQKIQNAYSKCKEYSDDKVQLAMQTYEMVDKHIRRLDADLARFEADLKDKMEGSDFESSGGRGLKKGRGQKEKRGSRGRGRRTSEEDTPKKKKHKGGSEFTDTILSVHPSDVLDMPVDPNEPTYCLCHQVSYGEMIGCDNPDCPIEWFHFACVDLTTKPKGK Predict reactive species |
| Full Name | inhibitor of growth family, member 5 |
| Calculated Molecular Weight | 28 kDa |
| Observed Molecular Weight | 28-32 kDa |
| GenBank Accession Number | BC005370 |
| Gene Symbol | ING5 |
| Gene ID (NCBI) | 84289 |
| RRID | AB_2280522 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8WYH8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ING5, also named as p28ING5, belongs to the ING family. It is a component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. ING5 is a component of the MOZ/MORF complex which has a histone H3 acetyltransferase activity. Through chromatin acetylation it may regulate DNA replication and may function as a transcriptional coactivator. (PMID:12750254) Altered ING5 expression and its shift into the cytoplasm may have an impact on the malignant transformation of colorectal epithelial cells and should be considered as a biomarker for colorectal carcinogenesis. (PMID:21193223)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ING5 antibody 10665-1-AP | Download protocol |
| IP protocol for ING5 antibody 10665-1-AP | Download protocol |
| WB protocol for ING5 antibody 10665-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ING5 Polyclonal antibody (Cat# 10665-1-AP) has 28 citations published in journals including Oncotarget, Mol Cancer, Cell Res, Cancer Lett, Front Med, EMBO Rep, MBio, Hum Pathol, Front Oncol, Thorac Cancer, Pancreas, Stem Cells Dev, J Proteome Res, Exp Cell Res, Cell Biol Int, J Cancer Res Clin Oncol, and Acta Biochim Biophys Sin. Research fields span multiple cancers (breast, lung, gastric, colorectal, bladder, ovarian, prostate, pancreatic, glioma), epigenetics, cardiomyocyte regeneration, obesity, viral infections, and stem cell biology.
| Species | Application | Title |
|---|---|---|
Cell Res LRP6 downregulation promotes cardiomyocyte proliferation and heart regeneration.
| ||
Cancer Lett MiR-193a-3p promotes the multi-chemoresistance of bladder cancer by targeting the HOXC9 gene. | ||
Front Med ING5 inhibits aerobic glycolysis of lung cancer cells by promoting TIE1-mediated phosphorylation of pyruvate dehydrogenase kinase 1 at Y163 | ||
Cancer Cell Int MicroRNA-196b-5p promotes malignant progression of colorectal cancer by targeting ING5.
| ||
Oncotarget The miR-193a-3p-regulated ING5 gene activates the DNA damage response pathway and inhibits multi-chemoresistance in bladder cancer. |
Reviews
What customers say
The single review for this antibody reports a 4-star rating. At a 1:1000 dilution, the user observed a lower-than-expected signal in Western blot, though a detectable band was still visible. Immunoprecipitation did not work as-is, but the reviewer noted it may simply require further optimization. Overall, the feedback suggests the antibody performs adequately for Western blot with a modest signal, while IP applications may need additional troubleshooting.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Jessica (Verified Customer) (10-06-2025) | Lower signal in western blot at 1:1000, but still detectable/visible band. IP did not work, but may require some optimization
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