Validation Data Gallery
|Positive WB detected in||A549 cells, HepG2 cells, SGC-7901 cells, MCF-7 cells|
|Positive IHC detected in||human liver tissue, human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
The immunogen of 22115-1-AP is INSIG1 Fusion Protein expressed in E. coli.
|Host / Isotype||Rabbit / IgG|
|Immunogen||INSIG1 fusion protein Ag17420|
|Full Name||insulin induced gene 1|
|Calculated molecular weight||277 aa, 30 kDa|
|Observed molecular weight||28-31 kDa, 66-70 kDa|
|GenBank accession number||BC001880|
|Gene ID (NCBI)||3638|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Insulin-induced genes (INSIGs), which consist of two isoforms (INSIG1 and INSIG2), are representative endoplasmic reticulum (ER)-resident oxysterol-binding proteins (OSBPs). INSIG1 is part of negative regulatory feedback, acting as a brake on SREBP transcriptional function (PMID: 23919961). INSIG1 also has a secondary function mediating sterolaccelerated proteolytic degradation of HMG CoA reductase, thus impacting the mevalonate pathway. Suppression of INSIG1 function promotes hepatic lipid remodelling and restrains NASH (non-alcoholic steatohepatitis) progression(PMID: 33722690).
Nat Cell Biol
Extracellular matrix mechanical cues regulate lipid metabolism through Lipin-1 and SREBP.
Vitamin D deficiency exacerbates atypical antipsychotic-induced metabolic side effects in rats: Involvement of the INSIG/SREBP pathway.
Hypomorphic ASGR1 modulates lipid homeostasis via INSIG1-mediated SREBP signaling suppression.
Mol Nutr Food Res
(-)-Epicatechin regulates blood lipids and attenuates hepatic steatosis in rats fed high-fat diet(†).
A potential mechanism underlying atypical antipsychotics-induced lipid disturbances.
The gluconeogenic enzyme PCK1 phosphorylates INSIG1/2 for lipogenesis.