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  • KD/KO Validated

INSIG1 Polyclonal antibody

INSIG1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 22115-1-AP

Synonyms

CL 6, INSIG 1, INSIG1, insulin induced gene 1, Insulin induced gene 1 protein



Tested Applications

Positive WB detected inA549 cells, HepG2 cells, SGC-7901 cells, MCF-7 cells
Positive IHC detected inhuman liver tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22115-1-AP is INSIG1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen INSIG1 fusion protein Ag17420
Full Name insulin induced gene 1
Calculated molecular weight 277 aa, 30 kDa
Observed molecular weight 28-31 kDa, 66-70 kDa
GenBank accession numberBC001880
Gene symbol INSIG1
Gene ID (NCBI) 3638
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for INSIG1 antibody 22115-1-APDownload protocol
IHC protocol for INSIG1 antibody 22115-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nat Cell Biol

Extracellular matrix mechanical cues regulate lipid metabolism through Lipin-1 and SREBP.

Authors - Patrizia Romani
ratWB

Eur Neuropsychopharmacol

Vitamin D deficiency exacerbates atypical antipsychotic-induced metabolic side effects in rats: Involvement of the INSIG/SREBP pathway.

Authors - Ruili Dang
ratWB

Mol Nutr Food Res

(-)-Epicatechin regulates blood lipids and attenuates hepatic steatosis in rats fed high-fat diet(†).

Authors - Hui Cheng
ratWB

Transl Psychiatry

A potential mechanism underlying atypical antipsychotics-induced lipid disturbances.

Authors - H L Cai
humanWB,IF

Nature

The gluconeogenic enzyme PCK1 phosphorylates INSIG1/2 for lipogenesis.

Authors - Daqian Xu
  • KD Validated