IPO7 Monoclonal antibody

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  WB analysis using 67525-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67525-1-Ig (IPO7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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  WB analysis using 67525-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67525-1-Ig (IPO7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 67525-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67525-1-Ig (IPO7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67525-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67525-1-Ig (IPO7 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IPO7 Monoclonal Antibody for WB, IHC, ELISA

Cat No. 67525-1-Ig

Clone No.1E7C12

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

FLJ14581, Imp7, importin 7, IPO7, Ran binding protein 7, RANBP7

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Tested Applications

• Positive WB detected in: A549 cells, HEK-293 cells, U2OS cells, LNCaP cells, HeLa cells, HepG2 cells, K-562 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67525-1-Ig targets IPO7 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: IPO7 fusion protein Ag27257
• Full Name: importin 7
• Calculated Molecular Weight: 120 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: NM_006391.2
• Gene Symbol: IPO7
• Gene ID (NCBI): 10527
• RRID: AB_2882745
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O95373
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.