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  • KD/KO Validated

IQGAP1 Polyclonal antibody

IQGAP1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 22167-1-AP

Synonyms

HUMORFA01, IQGAP1, KIAA0051, p195, SAR1



Tested Applications

Positive WB detected inA549 cells, HepG2 cells, NIH/3T3 cells, SGC-7901 cells, HeLa cells, HEK-293 cells, HepG2 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22167-1-AP is IQGAP1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IQGAP1 fusion protein Ag17821
Full Name IQ motif containing GTPase activating protein 1
Calculated molecular weight 189 kDa
Observed molecular weight 190-195 kDa
GenBank accession numberBC151834
Gene symbol IQGAP1
Gene ID (NCBI) 8826
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IQGAP1 is a member of a family of scaffolding proteins that interact with signaling and structural molecules. It localizes to sites of cell-cell contact in epithelial cells and regulates distinct cellular processes including cell adhesion, cell migration, extracellular signals through interacting with numerous protein. Multiple studies have shown that IQGAP1 is up-regulated in many human malignancies, such as lung cancer, ovarian cancer, colon cancer, breast cancer, melanoma and HCC. And IQGAP1 plays a critical role in cancer cell invasion.

Protocols

Product Specific Protocols
WB protocol for IQGAP1 antibody 22167-1-APDownload protocol
IHC protocol for IQGAP1 antibody 22167-1-APDownload protocol
IF protocol for IQGAP1 antibody 22167-1-APDownload protocol
IP protocol for IQGAP1 antibody 22167-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Oncol Lett

Circadian locomotor output cycles kaput affects the proliferation and migration of breast cancer cells by regulating the expression of E-cadherin via IQ motif containing GTPase activating protein 1.

Authors - Xiaoxue Li
mouseWB

Biochem Biophys Res Commun

MAL2 interacts with IQGAP1 to promote pancreatic cancer progression by increasing ERK1/2 phosphorylation.

Authors - Bin Zhang
humanWB,IF

Front Cell Infect Microbiol

IQGAP1 Mediates Hcp1-Promoted Escherichia coli Meningitis by Stimulating the MAPK Pathway.

Authors - Mingna Zhao
  • KD Validated
humanWB

Oncotarget

Diet-induced hypercholesterolemia promotes androgen-independent prostate cancer metastasis via IQGAP1 and caveolin-1.

Authors - Hyeongsun Moon
humanWB

Theranostics

Anti-allergic drug azelastine suppresses colon tumorigenesis by directly targeting ARF1 to inhibit IQGAP1-ERK-Drp1-mediated mitochondrial fission.

Authors - Hui-Fang Hu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Joleen (Verified Customer) (06-16-2019)

The antibody recognizes IQGAP (top band) but also another band at a lower molecular weight.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1k
  • Cell Tissue Type: MDCK GII
IQGAP1 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:22167-1-AP)
FH

Chun-Chih (Verified Customer) (01-31-2019)

Works well in IP at recommended conc. Clear and strong band, 1mg protein lysate per condition.

  • Applications: Immunoprecipitation,
  • Cell Tissue Type: Human breast cancer cells