|Positive WB detected in||A549 cells, HepG2 cells, NIH/3T3 cells, SGC-7901 cells, HeLa cells, HEK-293 cells, HepG2 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human breast cancer tissue, human colon cancer tissue, human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
The immunogen of 22167-1-AP is IQGAP1 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||IQGAP1 fusion protein Ag17821|
|Full Name||IQ motif containing GTPase activating protein 1|
|Calculated molecular weight||189 kDa|
|Observed molecular weight||190-195 kDa|
|GenBank accession number||BC151834|
|Gene ID (NCBI)||8826|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
IQGAP1 is a member of a family of scaffolding proteins that interact with signaling and structural molecules. It localizes to sites of cell-cell contact in epithelial cells and regulates distinct cellular processes including cell adhesion, cell migration, extracellular signals through interacting with numerous protein. Multiple studies have shown that IQGAP1 is up-regulated in many human malignancies, such as lung cancer, ovarian cancer, colon cancer, breast cancer, melanoma and HCC. And IQGAP1 plays a critical role in cancer cell invasion.
|Product Specific Protocols|
|WB protocol for IQGAP1 antibody 22167-1-AP||Download protocol|
|IHC protocol for IQGAP1 antibody 22167-1-AP||Download protocol|
|IF protocol for IQGAP1 antibody 22167-1-AP||Download protocol|
|IP protocol for IQGAP1 antibody 22167-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Circadian locomotor output cycles kaput affects the proliferation and migration of breast cancer cells by regulating the expression of E-cadherin via IQ motif containing GTPase activating protein 1.
Biochem Biophys Res Commun
MAL2 interacts with IQGAP1 to promote pancreatic cancer progression by increasing ERK1/2 phosphorylation.
Front Cell Infect Microbiol
IQGAP1 Mediates Hcp1-Promoted Escherichia coli Meningitis by Stimulating the MAPK Pathway.
Diet-induced hypercholesterolemia promotes androgen-independent prostate cancer metastasis via IQGAP1 and caveolin-1.
Anti-allergic drug azelastine suppresses colon tumorigenesis by directly targeting ARF1 to inhibit IQGAP1-ERK-Drp1-mediated mitochondrial fission.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Joleen (Verified Customer) (06-16-2019)
The antibody recognizes IQGAP (top band) but also another band at a lower molecular weight.
Chun-Chih (Verified Customer) (01-31-2019)
Works well in IP at recommended conc. Clear and strong band, 1mg protein lysate per condition.