IQGAP2 Polyclonal antibody

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  WB analysis using 30692-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30692-1-AP (IQGAP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 30692-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 30692-1-AP (IQGAP2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 30692-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 30692-1-AP (IQGAP2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 30692-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 30692-1-AP (IQGAP2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 30692-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 30692-1-AP (IQGAP2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse stomach using 30692-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 30692-1-AP (IQGAP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse stomach using 30692-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 30692-1-AP (IQGAP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IQGAP2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 30692-1-AP

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

IQGAP 2

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Tested Applications

• Positive WB detected in: HepG2 cells, HuH-7 cells, Ramos cells, human peripheral blood platelets, mouse testis tissue
• Positive IHC detected in: human stomach tissue, human intrahepatic cholangiocarcinoma tissue, mouse stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30692-1-AP targets IQGAP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag33518

  Product name: Recombinant human IQGAP2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 519-727 aa of NM_006633

  Sequence: DEIQQAVDDANVDKDRAKQWVTLVVDVNQCLEGKKSSDILSVLKSSTSNANDIIPECADKYYDALVKAKELKSERVSSDGSWLKLNLHKKYDYYYNTDSKESSWVTPESCLYKESWLTGKEIEDIIEEVTVGYIRENIWSASEELLLRFQATSSGPILREEFEARKSFLHEQEENVVKIQAFWKGYKQRKEYMHRRQTFIDNTDSIVKI

• Full Name: IQ motif containing GTPase activating protein 2
• Calculated Molecular Weight: 181 kDa
• Observed Molecular Weight: 181 kDa
• GenBank Accession Number: NM_006633
• Gene Symbol: IQGAP2
• Gene ID (NCBI): 10788
• RRID: AB_3086388
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13576
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IQGAP2, a member of the IQGAP family, functions as a tumor suppressor in most of the cancers. IQGAP2 contains three IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. IQGAP2 interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility. It also acts as a tumor suppressor and has been found to play a role in regulating innate antiviral responses.

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Denis (Verified Customer) (10-04-2025)

Used in mouse hypothalamus lysates at 1:1000. Clean and specific bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse hypothalamus