IRF3 Recombinant antibody

IRF3 Recombinant Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG


Human, mouse, zabrafish







Cat no : 80519-1-RR


interferon regulatory factor 3, IRF 3, IRF3

Tested Applications

Positive WB detected inHeLa cells, THP-1 cells, HT-29 cells, RAW 264.7 cells, zebrafish tissue, HepG2 cells, Jurkat cells
Positive IHC detected inhuman breast cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

80519-1-RR targets IRF3 in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, zabrafish samples.

Tested Reactivity Human, mouse, zabrafish
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name interferon regulatory factor 3
Calculated molecular weight 47 kDa
Observed molecular weight 50-60 kDa
GenBank accession numberBC009395
Gene symbol IRF3
Gene ID (NCBI) 3661
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

The virul-induced expression of interferon(IFN) genes in infected cells implicate in the interplay of several constitutively expressed and virus-activated transcription factors. A family of IFN regulatory factors(IRFs) have been shown to has a role in the transcription of IFN genes as well as IFN-stimulated genes. IRF3 is a novel key transcriptional regulator of type I IFN-dependent immune responses and involves in the innate immune response against DNA and RNA viruses, by binding to the promoters of IFN. It located in the cytoplasm of uninfected cells in an inactive form, and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, could be phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes.


Product Specific Protocols
WB protocol for IRF3 antibody 80519-1-RRDownload protocol
IHC protocol for IRF3 antibody 80519-1-RRDownload protocol
IF protocol for IRF3 antibody 80519-1-RRDownload protocol
Standard Protocols
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