IRF3 Recombinant antibody

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  WB analysis using 80519-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80519-1-RR (IRF3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 80519-1-RR

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 80519-1-RR (IRF3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of THP-1 using 80519-1-RR

  THP-1 cells were subjected to SDS PAGE followed by western blot with 80519-1-RR (IRF3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-29 using 80519-1-RR

  HT-29 cells were subjected to SDS PAGE followed by western blot with 80519-1-RR (IRF3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of zebrafish using 80519-1-RR

  Zebrafish tissue were subjected to SDS PAGE followed by western blot with 80519-1-RR (IRF3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 80519-1-RR

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80519-1-RR (IRF3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 80519-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80519-1-RR (IRF3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 80519-1-RR

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80519-1-RR (IRF3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 80519-1-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using IRF3 antibody (80519-1-RR, Clone: 2H6 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

IRF3 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 80519-1-RR

Clone No.2H6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, zebrafish

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

IRF-3, IRF 3, interferon regulatory factor 3, 2H6

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Tested Applications

• Positive WB detected in: HeLa cells, THP-1 cells, HT-29 cells, RAW 264.7 cells, zebrafish tissue, HepG2 cells, Jurkat cells
• Positive IHC detected in: human breast cancer tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

80519-1-RR targets IRF3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, zebrafish samples.

• Tested Reactivity: human, mouse, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: interferon regulatory factor 3
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 50-60 kDa
• GenBank Accession Number: BC009395
• Gene Symbol: IRF3
• Gene ID (NCBI): 3661
• RRID: AB_2918899
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q14653
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The virul-induced expression of interferon(IFN) genes in infected cells implicate in the interplay of several constitutively expressed and virus-activated transcription factors. A family of IFN regulatory factors(IRFs) have been shown to has a role in the transcription of IFN genes as well as IFN-stimulated genes. IRF3 is a novel key transcriptional regulator of type I IFN-dependent immune responses and involves in the innate immune response against DNA and RNA viruses, by binding to the promoters of IFN. It located in the cytoplasm of uninfected cells in an inactive form, and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, could be phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes.